Male Wistar rats each received an i.p. injection of the ribosome-inactivating proteins ricin or saporin, or a Ber-H2 (anti-CD30)-saporin immunotoxin at a dose corresponding to three times the LD50 calculated for mice. Animals were killed 24, 48 or 72 h after treatment. Histological examination showed hepatic necrosis in all treated animals, although the sinusoidal lining was affected only in ricin-poisoned rats. The activities of xanthine dehydrogenase (D-form) and oxidase (O form) were determined spectrophotometrically in liver and serum samples. In ricin-treated animals the liver enzyme was progressively converted from the D- to the Oform, which accounted for more than 60% of total activity after 48 h of poisoning, whilst no change in the xanthine oxidase activity was found in the serum. In the liver of rats treated with free or Ber-H2-conjugated saporin, the D-form was more than 75%, as in normal animals. In the same animals the serum xanthine oxidase activity was up to three-fold control values. The determination of serum xanthine oxidase may prove helpful in the evaluation of liver damage in patients treated with immunotoxins. It may become a diagnostic tool for the differential diagnosis of liver diseases. © Springer-Verlag 1996.

Battelli M.G., Buonamici L., Polito L., Bolognesi A., Stirpe F. (1996). Hepatotoxicity of ricin, saporin or a saporin immunotoxin: Xanthine oxidase activity in rat liver and blood serum. VIRCHOWS ARCHIV, 427(5), 529-535 [10.1007/BF00199514].

Hepatotoxicity of ricin, saporin or a saporin immunotoxin: Xanthine oxidase activity in rat liver and blood serum

Battelli M. G.;Buonamici L.;Polito L.;Bolognesi A.;Stirpe F.
1996

Abstract

Male Wistar rats each received an i.p. injection of the ribosome-inactivating proteins ricin or saporin, or a Ber-H2 (anti-CD30)-saporin immunotoxin at a dose corresponding to three times the LD50 calculated for mice. Animals were killed 24, 48 or 72 h after treatment. Histological examination showed hepatic necrosis in all treated animals, although the sinusoidal lining was affected only in ricin-poisoned rats. The activities of xanthine dehydrogenase (D-form) and oxidase (O form) were determined spectrophotometrically in liver and serum samples. In ricin-treated animals the liver enzyme was progressively converted from the D- to the Oform, which accounted for more than 60% of total activity after 48 h of poisoning, whilst no change in the xanthine oxidase activity was found in the serum. In the liver of rats treated with free or Ber-H2-conjugated saporin, the D-form was more than 75%, as in normal animals. In the same animals the serum xanthine oxidase activity was up to three-fold control values. The determination of serum xanthine oxidase may prove helpful in the evaluation of liver damage in patients treated with immunotoxins. It may become a diagnostic tool for the differential diagnosis of liver diseases. © Springer-Verlag 1996.
1996
Battelli M.G., Buonamici L., Polito L., Bolognesi A., Stirpe F. (1996). Hepatotoxicity of ricin, saporin or a saporin immunotoxin: Xanthine oxidase activity in rat liver and blood serum. VIRCHOWS ARCHIV, 427(5), 529-535 [10.1007/BF00199514].
Battelli M.G.; Buonamici L.; Polito L.; Bolognesi A.; Stirpe F.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/956283
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