Background: In developing muscle, stimulation of mitochondrial biogenesis and mtDNA expansion occur with downregulation of deoxynucleotide synthesis. Results: siRNA silencing of mitochondrial thymidine or deoxyguanosine kinase impacts myotube differentiation causing depletion of mtDNA and of all four deoxynucleotides. Conclusion: Shortage of even a single deoxynucleotide may upset the regulation of all DNA precursors. Significance: Deoxynucleotide analysis in myotubes unveils unexpected outcomes of synthetic enzyme deficiencies. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
Frangini M., Franzolin E., Chemello F., Laveder P., Romualdi C., Bianchi V., et al. (2013). Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 288(8), 5624-5635 [10.1074/jbc.M112.441147].
Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes
Chemello F.;
2013
Abstract
Background: In developing muscle, stimulation of mitochondrial biogenesis and mtDNA expansion occur with downregulation of deoxynucleotide synthesis. Results: siRNA silencing of mitochondrial thymidine or deoxyguanosine kinase impacts myotube differentiation causing depletion of mtDNA and of all four deoxynucleotides. Conclusion: Shortage of even a single deoxynucleotide may upset the regulation of all DNA precursors. Significance: Deoxynucleotide analysis in myotubes unveils unexpected outcomes of synthetic enzyme deficiencies. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
