Background: In developing muscle, stimulation of mitochondrial biogenesis and mtDNA expansion occur with downregulation of deoxynucleotide synthesis. Results: siRNA silencing of mitochondrial thymidine or deoxyguanosine kinase impacts myotube differentiation causing depletion of mtDNA and of all four deoxynucleotides. Conclusion: Shortage of even a single deoxynucleotide may upset the regulation of all DNA precursors. Significance: Deoxynucleotide analysis in myotubes unveils unexpected outcomes of synthetic enzyme deficiencies. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes / Frangini M.; Franzolin E.; Chemello F.; Laveder P.; Romualdi C.; Bianchi V.; Rampazzo C.. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - ELETTRONICO. - 288:8(2013), pp. 5624-5635. [10.1074/jbc.M112.441147]
Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes
Chemello F.;
2013
Abstract
Background: In developing muscle, stimulation of mitochondrial biogenesis and mtDNA expansion occur with downregulation of deoxynucleotide synthesis. Results: siRNA silencing of mitochondrial thymidine or deoxyguanosine kinase impacts myotube differentiation causing depletion of mtDNA and of all four deoxynucleotides. Conclusion: Shortage of even a single deoxynucleotide may upset the regulation of all DNA precursors. Significance: Deoxynucleotide analysis in myotubes unveils unexpected outcomes of synthetic enzyme deficiencies. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
