Eggplant is one of the important solanaceous vegetable crops consumed in India and worldwide. Seeds from symptomatic eggplants showing little leaf disease were planted in an insect-proof greenhouse. The DNA of these seedlings and those of their progeny were tested by nested PCR on phytoplasma 16S rRNA gene. RFLP analysis of obtained amplicons allowed the identification of phytoplasmas enclosed in five ribosomal groups in the seedlings. Moreover, 16SrI and 16SrXII groups were found in both seedlings and their progeny, confirming seed transmission of phytoplasmas infecting eggplants. Phytoplasmas belonging to 16SrIII and 16SrVI ribosomal groups were detected in symptomatic eggplants collected in the same field in the next season, confirming transmission of phytoplasmas also through the presence of insect vectors.
Seed transmission of phytoplasmas infecting eggplants in India
Darabakula M.;Contaldo N.;Pacini F.;Bertaccini A.
2023
Abstract
Eggplant is one of the important solanaceous vegetable crops consumed in India and worldwide. Seeds from symptomatic eggplants showing little leaf disease were planted in an insect-proof greenhouse. The DNA of these seedlings and those of their progeny were tested by nested PCR on phytoplasma 16S rRNA gene. RFLP analysis of obtained amplicons allowed the identification of phytoplasmas enclosed in five ribosomal groups in the seedlings. Moreover, 16SrI and 16SrXII groups were found in both seedlings and their progeny, confirming seed transmission of phytoplasmas infecting eggplants. Phytoplasmas belonging to 16SrIII and 16SrVI ribosomal groups were detected in symptomatic eggplants collected in the same field in the next season, confirming transmission of phytoplasmas also through the presence of insect vectors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
