: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK) are two enzymes of the Calvin Benson cycle that stand out for some peculiar properties they have in common: (i) they both use the products of light reactions for catalysis (NADPH for GAPDH, ATP for PRK), (ii) they are both light-regulated through thioredoxins and (iii) they are both involved in the formation of regulatory supramolecular complexes in the dark or low photosynthetic conditions, with or without the regulatory protein CP12. In the complexes, enzymes are transiently inactivated but ready to recover full activity after complex dissociation. Fully active GAPDH and PRK are in large excess for the functioning of the Calvin-Benson cycle, but they can limit the cycle upon complex formation. Complex dissociation contributes to photosynthetic induction. CP12 also controls PRK concentration in model photosynthetic organisms like Arabidopsis thaliana and Chlamydomonas reinhardtii. The review combines in vivo and in vitro data into an integrated physiological view of the role of GAPDH and PRK dark complexes in the regulation of photosynthesis.

Dark complexes of the Calvin-Benson cycle in a physiological perspective / Gurrieri L.; Sparla F.; Zaffagnini M.; Trost P.. - In: SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY. - ISSN 1084-9521. - ELETTRONICO. - 155:(2024), pp. 48-58. [10.1016/j.semcdb.2023.03.002]

Dark complexes of the Calvin-Benson cycle in a physiological perspective

Gurrieri L.
Primo
Writing – Original Draft Preparation
;
Sparla F.;Zaffagnini M.
Writing – Review & Editing
;
Trost P.
2024

Abstract

: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK) are two enzymes of the Calvin Benson cycle that stand out for some peculiar properties they have in common: (i) they both use the products of light reactions for catalysis (NADPH for GAPDH, ATP for PRK), (ii) they are both light-regulated through thioredoxins and (iii) they are both involved in the formation of regulatory supramolecular complexes in the dark or low photosynthetic conditions, with or without the regulatory protein CP12. In the complexes, enzymes are transiently inactivated but ready to recover full activity after complex dissociation. Fully active GAPDH and PRK are in large excess for the functioning of the Calvin-Benson cycle, but they can limit the cycle upon complex formation. Complex dissociation contributes to photosynthetic induction. CP12 also controls PRK concentration in model photosynthetic organisms like Arabidopsis thaliana and Chlamydomonas reinhardtii. The review combines in vivo and in vitro data into an integrated physiological view of the role of GAPDH and PRK dark complexes in the regulation of photosynthesis.
2024
Dark complexes of the Calvin-Benson cycle in a physiological perspective / Gurrieri L.; Sparla F.; Zaffagnini M.; Trost P.. - In: SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY. - ISSN 1084-9521. - ELETTRONICO. - 155:(2024), pp. 48-58. [10.1016/j.semcdb.2023.03.002]
Gurrieri L.; Sparla F.; Zaffagnini M.; Trost P.
File in questo prodotto:
File Dimensione Formato  
2024 Gurrieri Trost SCDB.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione - Non opere derivate (CCBYND)
Dimensione 2.37 MB
Formato Adobe PDF
2.37 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/954416
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact