The performance of selected spectral ranges in the low-frequency (10-150 cm-1), mid-frequency (1350-1500 cm-1), and high-frequency (i.e., C-H stretching; 2800-2950 cm-1) domains of Raman spectroscopy was evaluated for the kinetic in situ analysis of lipid polymorphic transformations. Tristearin was used as the primary model lipid and its spray-congealed formulations containing 5% w/w isopropyl myristate (IM), oleic acid (OA), and ethyl oleate (EO) were used due to their ability to differentially modulate the tristearin phase transformation from metastable α-form to the stable β-form. The behavior of bulk samples was interrogated under different isothermal conditions (35, 40, 45 and 50 °C) with Raman microscopy providing complementary particulate-level information for specific conditions, including dispersed state within an aqueous environment. Overall, a clear rank order was observed between the lipid additives (IM > EO > OA) for accelerating the conversion to β-form, best exemplified by the low-frequency Raman (LFR) domain. This spectral range also showed superior characteristics over the more commonly utilized mid-frequency and C-H stretching domains to detect faster onset times for the polymorphic transformations that were attributed to its intrinsic structural sensitivity.

Pasquarella C., Bertoni S., Passerini N., Boyd B.J., Be̅rziņš K. (2023). Comparison of Low-, Mid-, and High-Frequency Raman Spectroscopy for an In Situ Kinetic Analysis of Lipid Polymorphic Transformations. CRYSTAL GROWTH & DESIGN, 23(11), 7947-7957 [10.1021/acs.cgd.3c00737].

Comparison of Low-, Mid-, and High-Frequency Raman Spectroscopy for an In Situ Kinetic Analysis of Lipid Polymorphic Transformations

Pasquarella C.;Bertoni S.
Secondo
;
Passerini N.;
2023

Abstract

The performance of selected spectral ranges in the low-frequency (10-150 cm-1), mid-frequency (1350-1500 cm-1), and high-frequency (i.e., C-H stretching; 2800-2950 cm-1) domains of Raman spectroscopy was evaluated for the kinetic in situ analysis of lipid polymorphic transformations. Tristearin was used as the primary model lipid and its spray-congealed formulations containing 5% w/w isopropyl myristate (IM), oleic acid (OA), and ethyl oleate (EO) were used due to their ability to differentially modulate the tristearin phase transformation from metastable α-form to the stable β-form. The behavior of bulk samples was interrogated under different isothermal conditions (35, 40, 45 and 50 °C) with Raman microscopy providing complementary particulate-level information for specific conditions, including dispersed state within an aqueous environment. Overall, a clear rank order was observed between the lipid additives (IM > EO > OA) for accelerating the conversion to β-form, best exemplified by the low-frequency Raman (LFR) domain. This spectral range also showed superior characteristics over the more commonly utilized mid-frequency and C-H stretching domains to detect faster onset times for the polymorphic transformations that were attributed to its intrinsic structural sensitivity.
2023
Pasquarella C., Bertoni S., Passerini N., Boyd B.J., Be̅rziņš K. (2023). Comparison of Low-, Mid-, and High-Frequency Raman Spectroscopy for an In Situ Kinetic Analysis of Lipid Polymorphic Transformations. CRYSTAL GROWTH & DESIGN, 23(11), 7947-7957 [10.1021/acs.cgd.3c00737].
Pasquarella C.; Bertoni S.; Passerini N.; Boyd B.J.; Be̅rziņš K.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/951433
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