An isocratic high-performance liquid chromatographic method has been developed for the simultaneous determination of disulfiram and bupropionin human plasma samples. Analyses were carried out on aC8 reversed-phase column using a mobile phase composedof 50% acetonitrile and 50% aqueous phosphate buffer, containing triethylamine. Diode-array detection was used, operating at a wavelength of 250 nm. For the clean-up of plasma samples, a solid phase extraction procedure, based onC2 cartridges, was implemented. Extraction yields of the analytes were satisfactory, being always higher than 84%. The calibration curve was linear over the 5–500 ngmL-1 plasma concentration range for both disulfiram and bupropion. The method showed a high sensitivity (limit of detection of 1.5 ngmL-1) and satisfactory precision, selectivity and accuracy. The application to human plasma samples obtained from some alcohol and nicotine abusers also gave good results.
M.A. Saracino, C. Marcheselli, L. Somaini, G. Gerra, F. De Stefano, M.C. Pieri, et al. (2010). Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 398, 2155-2161 [10.1007/s00216-010-4172-z].
Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers
SARACINO, MARIA ADDOLORATA;MARCHESELLI, CHIARA;RAGGI, MARIA AUGUSTA
2010
Abstract
An isocratic high-performance liquid chromatographic method has been developed for the simultaneous determination of disulfiram and bupropionin human plasma samples. Analyses were carried out on aC8 reversed-phase column using a mobile phase composedof 50% acetonitrile and 50% aqueous phosphate buffer, containing triethylamine. Diode-array detection was used, operating at a wavelength of 250 nm. For the clean-up of plasma samples, a solid phase extraction procedure, based onC2 cartridges, was implemented. Extraction yields of the analytes were satisfactory, being always higher than 84%. The calibration curve was linear over the 5–500 ngmL-1 plasma concentration range for both disulfiram and bupropion. The method showed a high sensitivity (limit of detection of 1.5 ngmL-1) and satisfactory precision, selectivity and accuracy. The application to human plasma samples obtained from some alcohol and nicotine abusers also gave good results.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
