The role of Cpkk1, a mitogen-activated protein kinase from Crypho- nectria parasitica, was investigated by generating a number of mutant strains that overexpress, under the control of the cryparin promoter, both the wild-type protein and its allele with an extensive deletion in the catalytic domain. Furthermore, a hairpin construct was built and expressed to cause specific silencing of Cpkk1 mRNA transcripts. Specific mRNA silencing or overexpression was confirmed on both Northern and Western blot analysis. Selected C. parasitica strains with Cpkk1 either silenced or overexpressed were evaluated for their biological characteristics, including virulence on European chestnut, growth on different substrates, conidial sporulation, and resistance to cell- wall-degrading enzymes. Silencing of Cpkk1 and the overexpression of a defective Cpkk1 correlated with a marked reduction in virulence on 3-year-old chestnut trees, with no statistically significant effect on fungal growth in the various conditions tested.

L. Rostagno, A. Prodi, M. Turina (2010). Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut. PHYTOPATHOLOGY, 100, 1100-1110 [10.1094/PHYTO-02-10-0044].

Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut

PRODI, ANTONIO;
2010

Abstract

The role of Cpkk1, a mitogen-activated protein kinase from Crypho- nectria parasitica, was investigated by generating a number of mutant strains that overexpress, under the control of the cryparin promoter, both the wild-type protein and its allele with an extensive deletion in the catalytic domain. Furthermore, a hairpin construct was built and expressed to cause specific silencing of Cpkk1 mRNA transcripts. Specific mRNA silencing or overexpression was confirmed on both Northern and Western blot analysis. Selected C. parasitica strains with Cpkk1 either silenced or overexpressed were evaluated for their biological characteristics, including virulence on European chestnut, growth on different substrates, conidial sporulation, and resistance to cell- wall-degrading enzymes. Silencing of Cpkk1 and the overexpression of a defective Cpkk1 correlated with a marked reduction in virulence on 3-year-old chestnut trees, with no statistically significant effect on fungal growth in the various conditions tested.
2010
L. Rostagno, A. Prodi, M. Turina (2010). Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut. PHYTOPATHOLOGY, 100, 1100-1110 [10.1094/PHYTO-02-10-0044].
L. Rostagno; A. Prodi; M. Turina
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/94086
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 9
social impact