Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color” channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.

A. Bevilacqua, A. Gherardi, F. Piccinini (2010). Multichannel image mosaicing of stem cells. s.l : s.n.

Multichannel image mosaicing of stem cells

BEVILACQUA, ALESSANDRO;GHERARDI, ALESSANDRO;PICCININI, FILIPPO
2010

Abstract

Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color” channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.
2010
ICBSE 2010 Proceedings
254
257
A. Bevilacqua, A. Gherardi, F. Piccinini (2010). Multichannel image mosaicing of stem cells. s.l : s.n.
A. Bevilacqua; A. Gherardi; F. Piccinini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/94027
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