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EnglishImage mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color” channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.
| Titolo: | Multichannel image mosaicing of stem cells |
| Autore/i: | BEVILACQUA, ALESSANDRO; GHERARDI, ALESSANDRO; PICCININI, FILIPPO |
| Autore/i Unibo: | |
| Anno: | 2010 |
| Titolo del libro: | ICBSE 2010 Proceedings |
| Pagina iniziale: | 254 |
| Pagina finale: | 257 |
| Abstract: | Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color” channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes. |
| Data prodotto definitivo in UGOV: | 10-dic-2010 |
| Appare nelle tipologie: | 4.01 Contributo in Atti di convegno |
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