Background Clarithromycin (CH) and metronidazole (MZ) are routinely used in Helicobacter pylori treatment regimes. Recently, treatment with these antibiotics has been reported to fail in >30% of patients due to increasing resistance. The common chemosusceptibility methods are cumbersome and take up to 14 days. Chemosusceptibility of H pylori is therefore rarely checked. Aim To develop a rapid susceptibility test (RST) for H pylori. Methods Preliminary experiments were performed to find a broth medium able to support the growth of H pylori in 20 h and a reliable detection method to quickly detect the amount of H pylori grown in the media. A mixture of different components was the best broth medium; ELISA was chosen as the detection technique. Performance of the new RST was compared to a gold standard (break point agar dilution method). 200 consecutive patients were tested for H pylori infection and chemosusceptibility to CH and MZ by the gold standard and RST. Results 111 patients were infected with H pylori. 105 of these were positive by both RST and the gold standard culture method; 75/105 and 68/105 strains were susceptible to CH and MZ, respectively. RST misdiagnosed 2 strains of CH and 10 strains of MZ. Conclusions A new simple and rapid chemosusceptibility test for H pylori has been validated, which is simple to perform and reduces the procedure time from 6–14 days to 24 h

A new 24 h ELISA culture based method for Helicobacter pylori chemosusceptibility

PERNA, FEDERICO;VAIRA, BERARDINO
2010

Abstract

Background Clarithromycin (CH) and metronidazole (MZ) are routinely used in Helicobacter pylori treatment regimes. Recently, treatment with these antibiotics has been reported to fail in >30% of patients due to increasing resistance. The common chemosusceptibility methods are cumbersome and take up to 14 days. Chemosusceptibility of H pylori is therefore rarely checked. Aim To develop a rapid susceptibility test (RST) for H pylori. Methods Preliminary experiments were performed to find a broth medium able to support the growth of H pylori in 20 h and a reliable detection method to quickly detect the amount of H pylori grown in the media. A mixture of different components was the best broth medium; ELISA was chosen as the detection technique. Performance of the new RST was compared to a gold standard (break point agar dilution method). 200 consecutive patients were tested for H pylori infection and chemosusceptibility to CH and MZ by the gold standard and RST. Results 111 patients were infected with H pylori. 105 of these were positive by both RST and the gold standard culture method; 75/105 and 68/105 strains were susceptible to CH and MZ, respectively. RST misdiagnosed 2 strains of CH and 10 strains of MZ. Conclusions A new simple and rapid chemosusceptibility test for H pylori has been validated, which is simple to perform and reduces the procedure time from 6–14 days to 24 h
2010
Perna F; Vaira D.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/93693
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