A rapid and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of ribavirin (RBV) in rat brain was developed. Sample preparation required only two centrifuge steps before LC-MS/MS analysis and the chromatographic separation was achieved in isocratic conditions using an Atlantis T3 column with a nearly totally aqueous (95%) mobile phase. The method showed a good linearity over a concentration range of 5-1000 ppb and satisfactory results in terms of accuracy.
Zironi E., Gazzotti T., Lugoboni B., Barbarossa A., Scagliarini A., Pagliuca G. (2011). Development of a rapid LC-MS/MS method for ribavirun determination in rat brain. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 54, 889-892 [10.1016/j.jpba.2010.11.021].
Development of a rapid LC-MS/MS method for ribavirun determination in rat brain
ZIRONI, ELISA;GAZZOTTI, TERESA;LUGOBONI, BARBARA;BARBAROSSA, ANDREA;SCAGLIARINI, ALESSANDRA;PAGLIUCA, GIAMPIERO
2011
Abstract
A rapid and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of ribavirin (RBV) in rat brain was developed. Sample preparation required only two centrifuge steps before LC-MS/MS analysis and the chromatographic separation was achieved in isocratic conditions using an Atlantis T3 column with a nearly totally aqueous (95%) mobile phase. The method showed a good linearity over a concentration range of 5-1000 ppb and satisfactory results in terms of accuracy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
