Limited incorporation and modest bone remodeling can cause allograft failure. We investigated whether mesenchymal stem cells (MSCs) and osteogenic protein-1 (OP-1) can improve allograft integration. A 3-cm full-size intercalary bone defect was created in the mid-diaphysis of the metatarsal bone of the sheep and it was replaced with an allograft alone (control group), or with MSCs (MSC group), OP-1 (OP-1 group), or MSCs and OP-1 (MSC+OP-1 group). Radiographic results showed a faster and complete integration of the allograft in the MSC+OP-1 group. Histology demonstrated that the amount of new bone was significantly greater inside the graft and a longer vessel penetration in the MSC+OP-1 group than in others. Mechanical strength of the allograft was not compromised by the high rate of bone remodeling. These results demonstrated that the association of MSCs and OP-1 improve bone allograft integration promoting an almost complete bone restoring.

Osteogenic protein-1 associated with mesenchymal stem cells promote bone allograft integration.

DI BELLA, CLAUDIA;FRISONI, TOMMASO;DONATI, DAVIDE MARIA
2010

Abstract

Limited incorporation and modest bone remodeling can cause allograft failure. We investigated whether mesenchymal stem cells (MSCs) and osteogenic protein-1 (OP-1) can improve allograft integration. A 3-cm full-size intercalary bone defect was created in the mid-diaphysis of the metatarsal bone of the sheep and it was replaced with an allograft alone (control group), or with MSCs (MSC group), OP-1 (OP-1 group), or MSCs and OP-1 (MSC+OP-1 group). Radiographic results showed a faster and complete integration of the allograft in the MSC+OP-1 group. Histology demonstrated that the amount of new bone was significantly greater inside the graft and a longer vessel penetration in the MSC+OP-1 group than in others. Mechanical strength of the allograft was not compromised by the high rate of bone remodeling. These results demonstrated that the association of MSCs and OP-1 improve bone allograft integration promoting an almost complete bone restoring.
Di Bella C.; Aldini N.N.; Lucarelli E.; Dozza B.; Frisoni T.; Martini L.; Fini M.; Donati D.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/92597
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