The development and use of the single platform ISHAGE methodology allows direct quantification of the numbers of viable HPC transplanted: thus, the effects of sample handling, cryopreservation, storage and thawing on the recovery of CD34+ cells in any source of HPC (i.e. peripheral blood, apheresis product, bone marrow or umbilical cord blood) can be analysed. Furthermore, the analysis of subset composition od CD34+ cells in PBSC and marrow of poor mobilisers may allow a more mature definition of minimum graft size, as the precise phenotypes of the HPC responsible for short and long-term engraftment are still controversial.
Keeney M, Brown W, Gratama J, Papa S, Lanza F, Sutherland R (2003). Appendix 1: auto-standardization and compensation for CD34 analysis (Beckman-Coulter XL Flow Cytometer - System II Software). JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS, 17(3), 261-266.
Appendix 1: auto-standardization and compensation for CD34 analysis (Beckman-Coulter XL Flow Cytometer - System II Software)
Lanza FPenultimo
Membro del Collaboration Group
;
2003
Abstract
The development and use of the single platform ISHAGE methodology allows direct quantification of the numbers of viable HPC transplanted: thus, the effects of sample handling, cryopreservation, storage and thawing on the recovery of CD34+ cells in any source of HPC (i.e. peripheral blood, apheresis product, bone marrow or umbilical cord blood) can be analysed. Furthermore, the analysis of subset composition od CD34+ cells in PBSC and marrow of poor mobilisers may allow a more mature definition of minimum graft size, as the precise phenotypes of the HPC responsible for short and long-term engraftment are still controversial.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
