Glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts has been crystallized by vapour diffusion in the pH range 7-8.5 in (NH4)2SO4 and Tris-HCl buffer or potassium phosphate buffer at room temperature. Crystals of the A4 isoform, grown at pH 8.5 in Tris-HCl buffer, diffract to 3.0 Å (at 100 K) using synchrotron radiation. The crystals belong to the orthorhombic C222 space group, with unit-cell dimensions a = 145.9, b = 185.9 and c = 106.3 Å, and probably contain one tetramer per asymmetric unit. Structure determination by molecular replacement is in progress.
Crystallization and preliminary X-ray study of chloroplast glyceraldehyde-3-phosphate dehydrogenase / Sabatino P.; Fermani S.; Ripamonti A.; Cassetta A.; Scagliarini S.; Trost P.. - In: ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. - ISSN 0907-4449. - STAMPA. - 55:2(1999), pp. 566-567. [10.1107/S090744499801302X]
Crystallization and preliminary X-ray study of chloroplast glyceraldehyde-3-phosphate dehydrogenase
Sabatino P.
;Fermani S.;Ripamonti A.;Scagliarini S.;Trost P.
1999
Abstract
Glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts has been crystallized by vapour diffusion in the pH range 7-8.5 in (NH4)2SO4 and Tris-HCl buffer or potassium phosphate buffer at room temperature. Crystals of the A4 isoform, grown at pH 8.5 in Tris-HCl buffer, diffract to 3.0 Å (at 100 K) using synchrotron radiation. The crystals belong to the orthorhombic C222 space group, with unit-cell dimensions a = 145.9, b = 185.9 and c = 106.3 Å, and probably contain one tetramer per asymmetric unit. Structure determination by molecular replacement is in progress.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
