The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques.
Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections / Gatti, Giulia; Taddei, Francesca; Brandolini, Martina; Mancini, Andrea; Denicolò, Agnese; Congestrì, Francesco; Manera, Martina; Arfilli, Valentina; Battisti, Arianna; Zannoli, Silvia; Marino, Maria Michela; Marzucco, Anna; Morotti, Manuela; Grumiro, Laura; Scalcione, Agata; Dirani, Giorgio; Cricca, Monica; Sambri, Vittorio. - In: MICROORGANISMS. - ISSN 2076-2607. - STAMPA. - 10:8(2022), pp. 1573.1-1573.10. [10.3390/microorganisms10081573]
Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections
Gatti, Giulia;Brandolini, Martina;Congestrì, Francesco;Arfilli, Valentina;Marzucco, Anna;Grumiro, Laura;Dirani, Giorgio;Cricca, Monica;Sambri, Vittorio
2022
Abstract
The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques.File | Dimensione | Formato | |
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