The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques.

Molecular Approach for the Laboratory Diagnosis of Periprosthetic Joint Infections

Gatti, Giulia;Brandolini, Martina;Congestrì, Francesco;Arfilli, Valentina;Marzucco, Anna;Grumiro, Laura;Dirani, Giorgio;Cricca, Monica;Sambri, Vittorio
2022

Abstract

The incidence of total joint arthroplasty is increasing over time since the last decade and expected to be more than 4 million by 2030. As a consequence, the detection of infections associated with surgical interventions is increasing and prosthetic joint infections are representing both a clinically and economically challenging problem. Many pathogens, from bacteria to fungi, elicit the immune system response and produce a polymeric matrix, the biofilm, that serves as their protection. In the last years, the implementation of diagnostic methodologies reduced the error rate and the turn-around time: polymerase chain reaction, targeted or broad-spectrum, and next-generation sequencing have been introduced and they represent a robust approach nowadays that frees laboratories from the unique approach based on culture-based techniques.
2022
Gatti, Giulia; Taddei, Francesca; Brandolini, Martina; Mancini, Andrea; Denicolò, Agnese; Congestrì, Francesco; Manera, Martina; Arfilli, Valentina; Battisti, Arianna; Zannoli, Silvia; Marino, Maria Michela; Marzucco, Anna; Morotti, Manuela; Grumiro, Laura; Scalcione, Agata; Dirani, Giorgio; Cricca, Monica; Sambri, Vittorio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/919250
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