The need for single-camera 3D particle tracking methods is growing, among others, due to the increasing focus in biomedical research often relying on single-plane microscopy imaging. Defocusing-based methods are ideal for a wide-spread use as they rely on basic microscopy imaging rather than requiring additional non-standard optics. However, a wide-spread use has been limited by the lack of accessible and easy-to-use software. DefocusTracker is an open-source toolbox based on the universal principles of General Defocusing Particle Tracking (GDPT) relying solely on a reference look-up table and image recognition to connect a particle’s image and its respective out-of-plane depth coordinate. The toolbox is built in a modular fashion, allowing for easy addition of new image recognition methods, while maintaining the same workflow and external user interface. DefocusTracker is implemented in MATLAB, while a parallel implementation in Python is in the preparation.

DefocusTracker: A Modular Toolbox for Defocusing-based, Single-Camera, 3D Particle Tracking / Barnkob R.; Rossi M.. - In: JOURNAL OF OPEN RESEARCH SOFTWARE. - ISSN 2049-9647. - ELETTRONICO. - 9:22(2021), pp. 1-8. [10.5334/jors.351]

DefocusTracker: A Modular Toolbox for Defocusing-based, Single-Camera, 3D Particle Tracking

Rossi M.
Ultimo
2021

Abstract

The need for single-camera 3D particle tracking methods is growing, among others, due to the increasing focus in biomedical research often relying on single-plane microscopy imaging. Defocusing-based methods are ideal for a wide-spread use as they rely on basic microscopy imaging rather than requiring additional non-standard optics. However, a wide-spread use has been limited by the lack of accessible and easy-to-use software. DefocusTracker is an open-source toolbox based on the universal principles of General Defocusing Particle Tracking (GDPT) relying solely on a reference look-up table and image recognition to connect a particle’s image and its respective out-of-plane depth coordinate. The toolbox is built in a modular fashion, allowing for easy addition of new image recognition methods, while maintaining the same workflow and external user interface. DefocusTracker is implemented in MATLAB, while a parallel implementation in Python is in the preparation.
2021
DefocusTracker: A Modular Toolbox for Defocusing-based, Single-Camera, 3D Particle Tracking / Barnkob R.; Rossi M.. - In: JOURNAL OF OPEN RESEARCH SOFTWARE. - ISSN 2049-9647. - ELETTRONICO. - 9:22(2021), pp. 1-8. [10.5334/jors.351]
Barnkob R.; Rossi M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/918576
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