The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a pre-chromatographic reagent for LC analysis of amino acids is proposed. The reagent reacts (30 min at 50°C) with primary amino group and the resulting adducts can be chromatographed under reversed-phase conditions (Phenyl-Hexyl column). The experimental conditions for the derivatization and chromatographic separations are discussed. Application to the determination of several amino acids in pharmaceutical formulations is described.
4,7-Phenanthroline-5,6-dione: A new labelling reagent for liquid chromatographic analysis of amino acids
Gioia, M. G.;Gatti, R.
;Vannini, M.;HUDAIB, MOHAMMAD
2002
Abstract
The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a pre-chromatographic reagent for LC analysis of amino acids is proposed. The reagent reacts (30 min at 50°C) with primary amino group and the resulting adducts can be chromatographed under reversed-phase conditions (Phenyl-Hexyl column). The experimental conditions for the derivatization and chromatographic separations are discussed. Application to the determination of several amino acids in pharmaceutical formulations is described.File in questo prodotto:
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