The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a pre-chromatographic reagent for LC analysis of amino acids is proposed. The reagent reacts (30 min at 50°C) with primary amino group and the resulting adducts can be chromatographed under reversed-phase conditions (Phenyl-Hexyl column). The experimental conditions for the derivatization and chromatographic separations are discussed. Application to the determination of several amino acids in pharmaceutical formulations is described.
4,7-Phenanthroline-5,6-dione: A new labelling reagent for liquid chromatographic analysis of amino acids / Gioia, M.G.; Gatti, R.*; Vannini, M.; Hudaib, M.. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 56:5-6(2002), pp. 289-294. [10.1007/BF02491934]
4,7-Phenanthroline-5,6-dione: A new labelling reagent for liquid chromatographic analysis of amino acids
Gioia, M. G.;Gatti, R.
;Vannini, M.;HUDAIB, MOHAMMAD
2002
Abstract
The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a pre-chromatographic reagent for LC analysis of amino acids is proposed. The reagent reacts (30 min at 50°C) with primary amino group and the resulting adducts can be chromatographed under reversed-phase conditions (Phenyl-Hexyl column). The experimental conditions for the derivatization and chromatographic separations are discussed. Application to the determination of several amino acids in pharmaceutical formulations is described.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
