A molecular and serological survey was performed in order to investigate the role of some wild avian species in the epidemiology of Avian Pneumovirus (APV) infection. During 2001, 440 aquatic birds, including sea gulls, terns, herons, flamingos and shorebirds, were sampled in north-eastern wetlands of Italy. According to the taxonomic group, the sample sizes were: 179 Laridae, 161 Sternidae, 46 Ardeidae, 35 Phoenicopteridae, 19 Scolopacidae. Swabs from the choanal cleft were collected from 394 of these birds and tested by a subtype-specific RT-nested PCR, based on oligonucleotides specific for G gene sequence and able to differentiate A and B subtypes. At the same time, whenever possible, blood samples were collected (n. 368) and analysed using a blocking ELISA serological assay. All molecular and serological tests produced negative results.
Delogu M., De Marco M.A., CATELLI E. , CECCHINATO M. , Sperati Ruffoni L., Pesente P., et al. (2004). MOLECULAR AND SEROLOGICAL SURVEY OF AVIAN PNEUMOVIRUS INFECTION IN WILD AQUATIC BIRDS IN ITALY. WETTEMBERG : VVB LAUFERSWEILER VERLAG.
MOLECULAR AND SEROLOGICAL SURVEY OF AVIAN PNEUMOVIRUS INFECTION IN WILD AQUATIC BIRDS IN ITALY
DELOGU, MAURO;De Marco M. A.;CATELLI, ELENA;CECCHINATO, MATTIA;FRANCIOSI, CARLO
2004
Abstract
A molecular and serological survey was performed in order to investigate the role of some wild avian species in the epidemiology of Avian Pneumovirus (APV) infection. During 2001, 440 aquatic birds, including sea gulls, terns, herons, flamingos and shorebirds, were sampled in north-eastern wetlands of Italy. According to the taxonomic group, the sample sizes were: 179 Laridae, 161 Sternidae, 46 Ardeidae, 35 Phoenicopteridae, 19 Scolopacidae. Swabs from the choanal cleft were collected from 394 of these birds and tested by a subtype-specific RT-nested PCR, based on oligonucleotides specific for G gene sequence and able to differentiate A and B subtypes. At the same time, whenever possible, blood samples were collected (n. 368) and analysed using a blocking ELISA serological assay. All molecular and serological tests produced negative results.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
