The electrochemical reduction of CO2 to value-added products like formate represents a promising technology for the valorization of carbon dioxide. We propose a proof-of-concept bioelectrochemical system (BES) for the reduction of CO2 to formate. For the first time, our device employs a nanostructured titanium nitride (TiN) support for the immobilization of a formate dehydrogenase (FDH) enzyme. The hierarchical TiN nanostructured support exhibits high surface area and wide pore size distribution, achieving high catalytic loading, and is characterized by higher conductivity than other oxide-based supports employed for FDHs immobilization. We select the oxygen-tolerant FDH from Thiobacillus sp. KNK65MA (TsFDH) as enzymatic catalyst, which selectively reduces CO2 to formate. We identify an optimal TiN morphology for the enzyme immobilisation through enzymatic assay, reaching a catalyst loading of 59 mu g cm(-2) of specifically-adsorbed TsFDH and achieving a complete saturation of the anchoring sites available on the surface. We evaluate the electrochemical CO2 reduction performance of the TiN/TsFDH system, achieving a remarkable HCOO- Faradaic efficiency up to 76 %, a maximum formate yield of 44.1 mu mol mg(FDH)(-1) h(-1) and high stability. Our results show the technological feasibility of BES devices employing novel, nanostructured TiN-based supports, representing an important step in the optimization of these devices.

Arena, F., Giuffredi, G., Perego, A., Donini, S., Guzman, H., Hernandez, S., et al. (2021). Hierarchical TiN-Supported TsFDH Nanobiocatalyst for CO2 Reduction to Formate. CHEMELECTROCHEM, 8(15), 2846-2857 [10.1002/celc.202100480].

Hierarchical TiN-Supported TsFDH Nanobiocatalyst for CO2 Reduction to Formate

Parisini, E;
2021

Abstract

The electrochemical reduction of CO2 to value-added products like formate represents a promising technology for the valorization of carbon dioxide. We propose a proof-of-concept bioelectrochemical system (BES) for the reduction of CO2 to formate. For the first time, our device employs a nanostructured titanium nitride (TiN) support for the immobilization of a formate dehydrogenase (FDH) enzyme. The hierarchical TiN nanostructured support exhibits high surface area and wide pore size distribution, achieving high catalytic loading, and is characterized by higher conductivity than other oxide-based supports employed for FDHs immobilization. We select the oxygen-tolerant FDH from Thiobacillus sp. KNK65MA (TsFDH) as enzymatic catalyst, which selectively reduces CO2 to formate. We identify an optimal TiN morphology for the enzyme immobilisation through enzymatic assay, reaching a catalyst loading of 59 mu g cm(-2) of specifically-adsorbed TsFDH and achieving a complete saturation of the anchoring sites available on the surface. We evaluate the electrochemical CO2 reduction performance of the TiN/TsFDH system, achieving a remarkable HCOO- Faradaic efficiency up to 76 %, a maximum formate yield of 44.1 mu mol mg(FDH)(-1) h(-1) and high stability. Our results show the technological feasibility of BES devices employing novel, nanostructured TiN-based supports, representing an important step in the optimization of these devices.
2021
Arena, F., Giuffredi, G., Perego, A., Donini, S., Guzman, H., Hernandez, S., et al. (2021). Hierarchical TiN-Supported TsFDH Nanobiocatalyst for CO2 Reduction to Formate. CHEMELECTROCHEM, 8(15), 2846-2857 [10.1002/celc.202100480].
Arena, F; Giuffredi, G; Perego, A; Donini, S; Guzman, H; Hernandez, S; Stancanelli, E; Cosentino, C; Parisini, E; Di Fonzo, F
File in questo prodotto:
File Dimensione Formato  
Arena-ChemElectroChem-2021.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Creative commons
Dimensione 1.06 MB
Formato Adobe PDF
1.06 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/915509
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 8
social impact