Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and whole-genome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low clonality, evidence of genetic recombination, and high phenotypic diversity were detected. We show evidence that, although it is rare (possibly due to losses of sexual reproduction- and meiosis-associated genes) C. graminicola can undergo sexual recombination. Our results support the hypotheses that intra- and intercontinental pathogen migration and genetic recombination have great impacts on the C. graminicola population structure.

Population genomics provide insights into the global genetic structure of Colletotrichum graminicola, the causal agent of maize anthracnose / Rogério F, Baroncelli R, Cuevas-Fernández FB, Becerra S, Crouch JA, Bettiol W, Azcárate-Peril MA, Malapi-Wight M, Ortega V, Betran J, Tenuta A, Dambolena JS, Esker PD, Pedro Revilla, Jackson-Ziems TA, Hiltbrunner J, Munkvold G, Buhiniček I, Vicente-Villardón JL, Sukno SA, Thon MR. - In: MBIO. - ISSN 2161-2129. - ELETTRONICO. - 14:1(2023), pp. 1-18. [10.1128/mbio.02878-22]

Population genomics provide insights into the global genetic structure of Colletotrichum graminicola, the causal agent of maize anthracnose

Baroncelli R
Secondo
;
2023

Abstract

Understanding the genetic diversity and mechanisms underlying genetic variation in pathogen populations is crucial to the development of effective control strategies. We investigated the genetic diversity and reproductive biology of Colletotrichum graminicola isolates which infect maize by sequencing the genomes of 108 isolates collected from 14 countries using restriction site-associated DNA sequencing (RAD-seq) and whole-genome sequencing (WGS). Clustering analyses based on single-nucleotide polymorphisms revealed three genetic groups delimited by continental origin, compatible with short-dispersal of the pathogen and geographic subdivision. Intra- and intercontinental migration was observed between Europe and South America, likely associated with the movement of contaminated germplasm. Low clonality, evidence of genetic recombination, and high phenotypic diversity were detected. We show evidence that, although it is rare (possibly due to losses of sexual reproduction- and meiosis-associated genes) C. graminicola can undergo sexual recombination. Our results support the hypotheses that intra- and intercontinental pathogen migration and genetic recombination have great impacts on the C. graminicola population structure.
2023
Population genomics provide insights into the global genetic structure of Colletotrichum graminicola, the causal agent of maize anthracnose / Rogério F, Baroncelli R, Cuevas-Fernández FB, Becerra S, Crouch JA, Bettiol W, Azcárate-Peril MA, Malapi-Wight M, Ortega V, Betran J, Tenuta A, Dambolena JS, Esker PD, Pedro Revilla, Jackson-Ziems TA, Hiltbrunner J, Munkvold G, Buhiniček I, Vicente-Villardón JL, Sukno SA, Thon MR. - In: MBIO. - ISSN 2161-2129. - ELETTRONICO. - 14:1(2023), pp. 1-18. [10.1128/mbio.02878-22]
Rogério F, Baroncelli R, Cuevas-Fernández FB, Becerra S, Crouch JA, Bettiol W, Azcárate-Peril MA, Malapi-Wight M, Ortega V, Betran J, Tenuta A, Dambolena JS, Esker PD, Pedro Revilla, Jackson-Ziems TA, Hiltbrunner J, Munkvold G, Buhiniček I, Vicente-Villardón JL, Sukno SA, Thon MR
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/914701
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