A simple and reliable reversed-phase liquid chromatographic (RP-LC) method was developed and validated to determine simultaneously a glucosamine and chondroitin sulfate equivalent in dietary products. The procedure is based upon the reaction of o-phthaldialdehyde with glucosamine and galactosamine coming from the galactosaminoglycan hydrolysis. The hydrolysis reaction was carried out with hydrochloric acid (7.5 N) at 80°C for 8 hr, whereas, the pre-column derivatization reaction was carried out in alkaline media for 1 min at ambient temperature. The chromatographic separations were performed on a Phenomenex Synergi fusion-RP 80 A (250mm x 3.0 mm i.d.) using a mobile phase consisting of a mixture of sodium acetate buffer (pH 5.9; 0.05 M) and methanol (85:15, v/v). UV-DAD detection at 340 nm was used. Linear responses were observed and the limit of quantitation for both aminosaccharides was about 60 pmol. The intra-day precision (RSD) was about 1.8% and there was no significant difference between intra- and inter-day data. Recovery studies showed good results (99.3–101.0%) with RSD ranging from 1.1 to 2.1%.

R.Gatti*, P. Andreatta, M.G. Gioia, S. Boschetti (2010). A Simple and Validated LC Method for the Simultaneous Analysis of Glucosamine and Chondroitin Sulfate Equivalent in Dietary Products. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 33, 1760-1775 [10.1080/10826076.2010.526829].

A Simple and Validated LC Method for the Simultaneous Analysis of Glucosamine and Chondroitin Sulfate Equivalent in Dietary Products

GATTI, RITA;GIOIA, MARIA GRAZIA;
2010

Abstract

A simple and reliable reversed-phase liquid chromatographic (RP-LC) method was developed and validated to determine simultaneously a glucosamine and chondroitin sulfate equivalent in dietary products. The procedure is based upon the reaction of o-phthaldialdehyde with glucosamine and galactosamine coming from the galactosaminoglycan hydrolysis. The hydrolysis reaction was carried out with hydrochloric acid (7.5 N) at 80°C for 8 hr, whereas, the pre-column derivatization reaction was carried out in alkaline media for 1 min at ambient temperature. The chromatographic separations were performed on a Phenomenex Synergi fusion-RP 80 A (250mm x 3.0 mm i.d.) using a mobile phase consisting of a mixture of sodium acetate buffer (pH 5.9; 0.05 M) and methanol (85:15, v/v). UV-DAD detection at 340 nm was used. Linear responses were observed and the limit of quantitation for both aminosaccharides was about 60 pmol. The intra-day precision (RSD) was about 1.8% and there was no significant difference between intra- and inter-day data. Recovery studies showed good results (99.3–101.0%) with RSD ranging from 1.1 to 2.1%.
2010
R.Gatti*, P. Andreatta, M.G. Gioia, S. Boschetti (2010). A Simple and Validated LC Method for the Simultaneous Analysis of Glucosamine and Chondroitin Sulfate Equivalent in Dietary Products. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 33, 1760-1775 [10.1080/10826076.2010.526829].
R.Gatti*; P. Andreatta; M.G. Gioia; S. Boschetti
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/91400
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