Gene expression in mammalian cells can be suppressed by nucleic acid sequences complemetary to nedogenous transcripts. We employed thias strategy to block the sysnthesis of peptides derived from pro-opiomelanocortin in AtT-20 cells exposed to a 30-base oligodeoxynucleotide complementary to mRNA nucleotides which are translated into the first 10 aminoacids of beta-endorphin. A maximal reduction of immunoreactive beta-endorphine was detected in cell culture medium and in acetic acid extracts of AtT-20 cells exposed for 60 h to the antisense oligomer (10 and 100 nM).

Spampinato S., Canossa M., Carboni L., Campana G., Ferri S. (1994). Inhibition of proopiomelanocortin translation by an antisense oligodeoxynucleotide. REGULATORY PEPTIDES, 53(1), S141-S142 [10.1016/0167-0115(94)90281-X].

Inhibition of proopiomelanocortin translation by an antisense oligodeoxynucleotide

Spampinato S.;Canossa M.;Carboni L.;Campana G.;Ferri S.
1994

Abstract

Gene expression in mammalian cells can be suppressed by nucleic acid sequences complemetary to nedogenous transcripts. We employed thias strategy to block the sysnthesis of peptides derived from pro-opiomelanocortin in AtT-20 cells exposed to a 30-base oligodeoxynucleotide complementary to mRNA nucleotides which are translated into the first 10 aminoacids of beta-endorphin. A maximal reduction of immunoreactive beta-endorphine was detected in cell culture medium and in acetic acid extracts of AtT-20 cells exposed for 60 h to the antisense oligomer (10 and 100 nM).
1994
Spampinato S., Canossa M., Carboni L., Campana G., Ferri S. (1994). Inhibition of proopiomelanocortin translation by an antisense oligodeoxynucleotide. REGULATORY PEPTIDES, 53(1), S141-S142 [10.1016/0167-0115(94)90281-X].
Spampinato S.; Canossa M.; Carboni L.; Campana G.; Ferri S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/913226
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