Gene expression in mammalian cells can be suppressed by nucleic acid sequences complemetary to nedogenous transcripts. We employed thias strategy to block the sysnthesis of peptides derived from pro-opiomelanocortin in AtT-20 cells exposed to a 30-base oligodeoxynucleotide complementary to mRNA nucleotides which are translated into the first 10 aminoacids of beta-endorphin. A maximal reduction of immunoreactive beta-endorphine was detected in cell culture medium and in acetic acid extracts of AtT-20 cells exposed for 60 h to the antisense oligomer (10 and 100 nM).
Inhibition of proopiomelanocortin translation by an antisense oligodeoxynucleotide / Spampinato S.; Canossa M.; Carboni L.; Campana G.; Ferri S.. - In: REGULATORY PEPTIDES. - ISSN 0167-0115. - STAMPA. - 53:1(1994), pp. S141-S142. [10.1016/0167-0115(94)90281-X]
Inhibition of proopiomelanocortin translation by an antisense oligodeoxynucleotide
Spampinato S.;Canossa M.;Carboni L.;Campana G.;Ferri S.
1994
Abstract
Gene expression in mammalian cells can be suppressed by nucleic acid sequences complemetary to nedogenous transcripts. We employed thias strategy to block the sysnthesis of peptides derived from pro-opiomelanocortin in AtT-20 cells exposed to a 30-base oligodeoxynucleotide complementary to mRNA nucleotides which are translated into the first 10 aminoacids of beta-endorphin. A maximal reduction of immunoreactive beta-endorphine was detected in cell culture medium and in acetic acid extracts of AtT-20 cells exposed for 60 h to the antisense oligomer (10 and 100 nM).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
