Prazosin-related quinazolines 4-20 were synthesized, and their biological profiles at α1-adrenoreceptor subtypes were assessed by functional experiments in isolated rat vas deferens (α(1A)), spleen (α(1B)), and aorta (α(1D)) and by binding assays in CHO cells expressing human cloned α1-adrenoreceptor subtypes. The replacement of piperazine and furan units of prazosin (1) by 1,6-hexanediamine and phenyl moieties, respectively, affording 3-20, markedly affected both affinity and selectivity for α1-adrenoreceptor subtypes in functional experiments. Cystazosin (3), bearing a cystamine moiety, was a selective α(1D)-adrenoreceptor antagonist being 1 order of magnitude more potent at α(1D)-adrenoreceptors (pA2, 8.54 ± 0.02) than at the α(1A)-(pA2, 7.53 ± 0.01) and α(1B)-subtypes (pA2, 7.49 ± 0.01). The insertion of substituents on the furan ring of 3, as in compounds 4 and 5, did not improve the selectivity profile. The simultaneous replacement of both piperazine and furan rings of 1 gave 8 which resulted in a potent, selective α(1B)-adrenoreceptor antagonist (85- and 15-fold more potent than at α(1A)- and α(1D)-subtypes, respectively). The insertion of substituents on the benzene ring of 8 affected, according to the type and the position of the substituent, affinity and selectivity for α1- adrenoreceptors. Consequently, the insertion of appropriate substituents in the phenyl ring of 8 may represent the basis of designing new selective ligands for α1-adrenoreceptor subtypes. Interestingly, the finding that polyamines 11, 16, and 20, bearing a 1,6-hexanediamine moiety, retained high affinity for α1-adrenoreceptor subtypes suggests that the substituent did not give rise to negative interactions with the receptor. Finally, binding assays performed with selected quinazolines (2, 3, and 14) produced affinity results, which were not in agreement with the selectivity profiles obtained from functional experiments. This rather surprising and unexpected finding may be explained by considering neutral and negative antagonism.
Bolognesi M.L., Budriesi R., Chiarini A., Poggesi E., Leonardi A., Melchiorre C. (1998). Design, synthesis, and biological activity of prazosin-related antagonists. Role of the piperazine and furan units of prazosin on the selectivity for α1-adrenoreceptor subtypes. JOURNAL OF MEDICINAL CHEMISTRY, 41(24), 4844-4853 [10.1021/jm9810654].
Design, synthesis, and biological activity of prazosin-related antagonists. Role of the piperazine and furan units of prazosin on the selectivity for α1-adrenoreceptor subtypes
Bolognesi M. L.;Budriesi R.;Chiarini A.;Melchiorre C.
1998
Abstract
Prazosin-related quinazolines 4-20 were synthesized, and their biological profiles at α1-adrenoreceptor subtypes were assessed by functional experiments in isolated rat vas deferens (α(1A)), spleen (α(1B)), and aorta (α(1D)) and by binding assays in CHO cells expressing human cloned α1-adrenoreceptor subtypes. The replacement of piperazine and furan units of prazosin (1) by 1,6-hexanediamine and phenyl moieties, respectively, affording 3-20, markedly affected both affinity and selectivity for α1-adrenoreceptor subtypes in functional experiments. Cystazosin (3), bearing a cystamine moiety, was a selective α(1D)-adrenoreceptor antagonist being 1 order of magnitude more potent at α(1D)-adrenoreceptors (pA2, 8.54 ± 0.02) than at the α(1A)-(pA2, 7.53 ± 0.01) and α(1B)-subtypes (pA2, 7.49 ± 0.01). The insertion of substituents on the furan ring of 3, as in compounds 4 and 5, did not improve the selectivity profile. The simultaneous replacement of both piperazine and furan rings of 1 gave 8 which resulted in a potent, selective α(1B)-adrenoreceptor antagonist (85- and 15-fold more potent than at α(1A)- and α(1D)-subtypes, respectively). The insertion of substituents on the benzene ring of 8 affected, according to the type and the position of the substituent, affinity and selectivity for α1- adrenoreceptors. Consequently, the insertion of appropriate substituents in the phenyl ring of 8 may represent the basis of designing new selective ligands for α1-adrenoreceptor subtypes. Interestingly, the finding that polyamines 11, 16, and 20, bearing a 1,6-hexanediamine moiety, retained high affinity for α1-adrenoreceptor subtypes suggests that the substituent did not give rise to negative interactions with the receptor. Finally, binding assays performed with selected quinazolines (2, 3, and 14) produced affinity results, which were not in agreement with the selectivity profiles obtained from functional experiments. This rather surprising and unexpected finding may be explained by considering neutral and negative antagonism.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
