An in-vitro model of human bone marrow mesenchymal stem cells (hBM-MSCs) myogenic commitment by synergic effect of a differentiation media coupled with human primary skeletal myoblasts (hSkMs) co-culture was developed adopting both conventional static co-seeding and perfused culture systems. Static co-seeding provided a notable outcome in terms of gene expression with a significant increase of Desmin (141-fold) and Myosin heavy chain II (MYH2, 32-fold) at day 21, clearly detected also by semi-quantitative immunofluorescence. Under perfusion conditions, myogenic induction ability of hSkMs on hBM-MSCs was exerted by paracrine effect with an excellent gene overexpression and immunofluorescence detection of MYH2 protein; furthermore, due to the dynamic cell culture in separate wells, western blot data were acquired confirming a successful cell commitment at day 14. A significant increase of anti-inflammatory cytokine gene expression, including IL-10 and IL-4 (15-fold and 11-fold, respectively) at day 14, with respect to the pro-inflammatory cytokines IL-12A (7-fold at day 21) and IL-1 beta (1.4-fold at day 7) was also detected during dynamic culture, confirming the immunomodulatory activity of hBM-MSCs along with commitment events. The present study opens interesting perspectives on the use of dynamic culture based on perfusion as a versatile tool to study myogenic events and paracrine cross-talk compared to the simple co-seeding static culture.

Myogenic commitment of human stem cells by myoblasts Co-culture: a static vs. a dynamic approach / Scala, Pasqualina; Lovecchio, J; Lamparelli, E P; Vitolo, R; Giudice, V; Giordano, E; Selleri, C; Rehak, L; Maffulli, N; Della Porta, G. - In: ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY. - ISSN 2169-1401. - ELETTRONICO. - 50:1(2022), pp. 49-58. [10.1080/21691401.2022.2039684]

Myogenic commitment of human stem cells by myoblasts Co-culture: a static vs. a dynamic approach

Lovecchio, J;Giordano, E;
2022

Abstract

An in-vitro model of human bone marrow mesenchymal stem cells (hBM-MSCs) myogenic commitment by synergic effect of a differentiation media coupled with human primary skeletal myoblasts (hSkMs) co-culture was developed adopting both conventional static co-seeding and perfused culture systems. Static co-seeding provided a notable outcome in terms of gene expression with a significant increase of Desmin (141-fold) and Myosin heavy chain II (MYH2, 32-fold) at day 21, clearly detected also by semi-quantitative immunofluorescence. Under perfusion conditions, myogenic induction ability of hSkMs on hBM-MSCs was exerted by paracrine effect with an excellent gene overexpression and immunofluorescence detection of MYH2 protein; furthermore, due to the dynamic cell culture in separate wells, western blot data were acquired confirming a successful cell commitment at day 14. A significant increase of anti-inflammatory cytokine gene expression, including IL-10 and IL-4 (15-fold and 11-fold, respectively) at day 14, with respect to the pro-inflammatory cytokines IL-12A (7-fold at day 21) and IL-1 beta (1.4-fold at day 7) was also detected during dynamic culture, confirming the immunomodulatory activity of hBM-MSCs along with commitment events. The present study opens interesting perspectives on the use of dynamic culture based on perfusion as a versatile tool to study myogenic events and paracrine cross-talk compared to the simple co-seeding static culture.
2022
Myogenic commitment of human stem cells by myoblasts Co-culture: a static vs. a dynamic approach / Scala, Pasqualina; Lovecchio, J; Lamparelli, E P; Vitolo, R; Giudice, V; Giordano, E; Selleri, C; Rehak, L; Maffulli, N; Della Porta, G. - In: ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY. - ISSN 2169-1401. - ELETTRONICO. - 50:1(2022), pp. 49-58. [10.1080/21691401.2022.2039684]
Scala, Pasqualina; Lovecchio, J; Lamparelli, E P; Vitolo, R; Giudice, V; Giordano, E; Selleri, C; Rehak, L; Maffulli, N; Della Porta, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/910759
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