In this work the aerobic cometabolic biodegradation of 1,1,2,2-tetrachloroethane (TeCA) by propane-utilizing bacteria was studied in slurry bioreactors containing soil and groundwater from 5 aquifers as well as in soil-free bioreactors. The main goals were: a) to identify and calibrate a kinetic model of TeCA cometabolism; b) to select and characterize a TeCA-degrading bacterial consortium; c) to compare the results obtained in slurry and in soil-free bioreactors. The results showed that 4 of the 5 tested aquifers contain TeCA-degrading bacteria, indicating that aerobic cometabolism is a potentially effective approach for TeCA-contaminated aquifers. In bioaugmentation tests, a TeCA-cometabolizing consortium developed in the slurry bioreactors induced a strong reduction of the lag-time for the onset of TeCA cometabolism. The soil-free tests yielded a satisfactory TeCA degradation performance, indicating that on-site soil-free bioreactors represent an interesting technical solution for the aerobic cometabolic bioremediation of CAH-contaminated groundwaters. The mineralization of the organic Cl was equal to about 97%. The prolonged TeCA biodegradation determined a progressive selection of the bacterial strains more effective in TeCA degradation and less affected by degradation product toxicity. The tested Michaelis-Menten-based kinetic model proved an effective tool to interpret the experimental data of TeCA aerobic cometabolism.

D. Frascari, M. Cappelletti, S. Fedi, D. Zannoni, M. Nocentini, D. Pinelli (2010). 1,1,2,2-Tetrachloroethane aerobic cometabolic biodegradation in slurry and soil-free bioreactors: a kinetic study. BIOCHEMICAL ENGINEERING JOURNAL, 52, 55-64 [10.1016/j.bej.2010.07.004].

1,1,2,2-Tetrachloroethane aerobic cometabolic biodegradation in slurry and soil-free bioreactors: a kinetic study

FRASCARI, DARIO;CAPPELLETTI, MARTINA;FEDI, STEFANO;ZANNONI, DAVIDE;NOCENTINI, MASSIMO;PINELLI, DAVIDE
2010

Abstract

In this work the aerobic cometabolic biodegradation of 1,1,2,2-tetrachloroethane (TeCA) by propane-utilizing bacteria was studied in slurry bioreactors containing soil and groundwater from 5 aquifers as well as in soil-free bioreactors. The main goals were: a) to identify and calibrate a kinetic model of TeCA cometabolism; b) to select and characterize a TeCA-degrading bacterial consortium; c) to compare the results obtained in slurry and in soil-free bioreactors. The results showed that 4 of the 5 tested aquifers contain TeCA-degrading bacteria, indicating that aerobic cometabolism is a potentially effective approach for TeCA-contaminated aquifers. In bioaugmentation tests, a TeCA-cometabolizing consortium developed in the slurry bioreactors induced a strong reduction of the lag-time for the onset of TeCA cometabolism. The soil-free tests yielded a satisfactory TeCA degradation performance, indicating that on-site soil-free bioreactors represent an interesting technical solution for the aerobic cometabolic bioremediation of CAH-contaminated groundwaters. The mineralization of the organic Cl was equal to about 97%. The prolonged TeCA biodegradation determined a progressive selection of the bacterial strains more effective in TeCA degradation and less affected by degradation product toxicity. The tested Michaelis-Menten-based kinetic model proved an effective tool to interpret the experimental data of TeCA aerobic cometabolism.
2010
D. Frascari, M. Cappelletti, S. Fedi, D. Zannoni, M. Nocentini, D. Pinelli (2010). 1,1,2,2-Tetrachloroethane aerobic cometabolic biodegradation in slurry and soil-free bioreactors: a kinetic study. BIOCHEMICAL ENGINEERING JOURNAL, 52, 55-64 [10.1016/j.bej.2010.07.004].
D. Frascari; M. Cappelletti; S. Fedi; D. Zannoni; M. Nocentini; D. Pinelli
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/90977
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