Until now only planar projections of the molecular shape of supercoiled circular DNA molecules could be imaged with scanning force microscopy (SFM). In the three‐dimensional image of a supercoiled pBR322 DNA molecule in this report, the chirality and writhing number can be “read” directly. Local changes in the supercoiling in individual DNA molecules can be monitored, which should be important for our understanding of biological processes such as DNA transcription. Copyright © 1993 by VCH Verlagsgesellschaft mbH, Germany
DNA Supercoiling Imaged in Three Dimensions by Scanning Force Microscopy / Bruno Samorì*, Carmelo Nigro, Valerio Armentario, Salvatore Cimieri, Giampaolo Zuccheri, Carla Quagliariello. - In: ANGEWANDTE CHEMIE. INTERNATIONAL EDITION IN ENGLISH. - ISSN 0570-0833. - STAMPA. - 32:10(1993), pp. 1461-1463. [10.1002/anie.199314611]
DNA Supercoiling Imaged in Three Dimensions by Scanning Force Microscopy
Giampaolo ZuccheriPenultimo
Investigation
;
1993
Abstract
Until now only planar projections of the molecular shape of supercoiled circular DNA molecules could be imaged with scanning force microscopy (SFM). In the three‐dimensional image of a supercoiled pBR322 DNA molecule in this report, the chirality and writhing number can be “read” directly. Local changes in the supercoiling in individual DNA molecules can be monitored, which should be important for our understanding of biological processes such as DNA transcription. Copyright © 1993 by VCH Verlagsgesellschaft mbH, GermanyI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
