We have previously documented a dramatic elevation in the activity of alpha 2,6-sialytransferase towards Gal beta 1,4GlcNAc (EC 2.4.99.1) (alpha 2,6ST) in CaCo-2 cells maintained in culture for several days after confluence to elicit a high degree of enterocytic differentiation phenotype. Northern analysis performed with a probe complementary to a region of human alpha 2,6ST mRNA common to all known transcripts demonstrated that the expression of alpha 2,6ST mRNA in CaCo-2 cells increased with the degree with the degree of differentiation. When probes complementary to 5'-untranslated exons (Y + Z or X) previously identified in transcripts isolated from human placenta and from several human lymphoblastoid cell lines were used, no hybridization signal with mRNA of CaCo-2 cells was found, as reported for the mRNA of hepatoma cell line HepG2 (Wang XC, Vertino A, Eddy RL, Byers MG, Jani-Sait SN, Shows TB, Lau JTY (1993) J Biol Chem 268: 4355-61). These results support the notion that the major alpha 2,6ST transcript of CaCo-2 cells was the hepatoma isoform or a new one, so far unreported. Consistent with the differentiation-dependent increase in alpha 2,6ST- mRNA expression, an elevation of the reactivity with Sambucus nigra agglutinin of differentiated CaCo-2 cell-surface was observed, indicating an enhanced alpha 2,6-sialylation of membrane glycoconjugates

Differentiation-dependent expression of human beta-galactoside alpha2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells / Fabio Dall'olio; Nadia Malagolini; Stefania Guerrini; Joseph T. Y. Lau; Franca Serafini. - In: GLYCOCONJUGATE JOURNAL. - ISSN 0282-0080. - STAMPA. - 13:1(1996), pp. 115-121. [10.1007/bf01049687]

Differentiation-dependent expression of human beta-galactoside alpha2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells

Fabio Dall'olio;Nadia Malagolini;Stefania Guerrini;Franca Serafini
1996

Abstract

We have previously documented a dramatic elevation in the activity of alpha 2,6-sialytransferase towards Gal beta 1,4GlcNAc (EC 2.4.99.1) (alpha 2,6ST) in CaCo-2 cells maintained in culture for several days after confluence to elicit a high degree of enterocytic differentiation phenotype. Northern analysis performed with a probe complementary to a region of human alpha 2,6ST mRNA common to all known transcripts demonstrated that the expression of alpha 2,6ST mRNA in CaCo-2 cells increased with the degree with the degree of differentiation. When probes complementary to 5'-untranslated exons (Y + Z or X) previously identified in transcripts isolated from human placenta and from several human lymphoblastoid cell lines were used, no hybridization signal with mRNA of CaCo-2 cells was found, as reported for the mRNA of hepatoma cell line HepG2 (Wang XC, Vertino A, Eddy RL, Byers MG, Jani-Sait SN, Shows TB, Lau JTY (1993) J Biol Chem 268: 4355-61). These results support the notion that the major alpha 2,6ST transcript of CaCo-2 cells was the hepatoma isoform or a new one, so far unreported. Consistent with the differentiation-dependent increase in alpha 2,6ST- mRNA expression, an elevation of the reactivity with Sambucus nigra agglutinin of differentiated CaCo-2 cell-surface was observed, indicating an enhanced alpha 2,6-sialylation of membrane glycoconjugates
1996
Differentiation-dependent expression of human beta-galactoside alpha2,6-sialyltransferase mRNA in colon carcinoma CaCo-2 cells / Fabio Dall'olio; Nadia Malagolini; Stefania Guerrini; Joseph T. Y. Lau; Franca Serafini. - In: GLYCOCONJUGATE JOURNAL. - ISSN 0282-0080. - STAMPA. - 13:1(1996), pp. 115-121. [10.1007/bf01049687]
Fabio Dall'olio; Nadia Malagolini; Stefania Guerrini; Joseph T. Y. Lau; Franca Serafini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/903832
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