The presence of phytoplasma in Fragaria ananassa x Duch cv Senga Sengana showing strawberry green petals symptoms was observed by electron microscopy of phloem tissue. No phytoplasmas were found in asymptomatic strawberry plants used as controls. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rRNA sequences specific for phytoplasmas. Bands of 1.2 kb were obtained and the subsequent nested-PCR with specific primers and RFLP analyses allowed to classify the detected phytoplasmas in the aster yellows group (16SrI). They belonged to the subgroup I-C of which type strain is clover phyllody phytoplasma.
Electron microscopy and molecular identification of phytoplasmas associated with strawberry green petals in the Czech Republic / Honetslegrova J.F.; Vibio M.; Bertaccini A.. - In: EUROPEAN JOURNAL OF PLANT PATHOLOGY. - ISSN 0929-1873. - STAMPA. - 102:9(1996), pp. 831-835. [10.1007/BF01877052]
Electron microscopy and molecular identification of phytoplasmas associated with strawberry green petals in the Czech Republic
Vibio M.;Bertaccini A.
1996
Abstract
The presence of phytoplasma in Fragaria ananassa x Duch cv Senga Sengana showing strawberry green petals symptoms was observed by electron microscopy of phloem tissue. No phytoplasmas were found in asymptomatic strawberry plants used as controls. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rRNA sequences specific for phytoplasmas. Bands of 1.2 kb were obtained and the subsequent nested-PCR with specific primers and RFLP analyses allowed to classify the detected phytoplasmas in the aster yellows group (16SrI). They belonged to the subgroup I-C of which type strain is clover phyllody phytoplasma.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
