Flavescence dorée is a devastating disease of grapevine widespread in several countries in EU such as France, Italy and Spain. Genetic variability among 17 Italian and 3 French FD strains was investigated by RFLP analyses based on a fragment of the ribosomal protein operon and on the non-ribosomal DNA fragment FD9. RFLP analysis of the PCR amplified ribosomal protein fragment, coding for the 3′ end of rp122 and the entire rps3 genes, differentiated 4 rp-subgroups among the FD strains and 4 subgroups among the reference strains belonging to elm yellows group (16SrV). Sequencing and phylogenetic analysis of the same ribosomal protein DNA fragment validated the delineation of 4 distinct FD strain types derived by RFLP analyses. The results supported the differentiation based on analysis of the non-ribosomal DNA fragment FD9. The phylogenetic analysis further revealed relationships and a probable evolutionary trend among the FD strains and the other representatives of elm yellows group. All the FD strains together with the reference strains ALY, RuS and JWB formed a cluster very well distinct from the EY/ULW cluster. Moreover, ALY was shown to be more closely related to three FD strain types: the Lombardia/Piemonte, the French FD70, and the French FD88/Italian FD-D strain clusters. © 2002 Elsevier Science Ltd. All rights reserved.

Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France / Martini M.; Botti S.; Marcone C.; Marzachi C.; Casati P.; Bianco P.A.; Benedetti R.; Bertaccini A.. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - STAMPA. - 16:3(2002), pp. 197-208. [10.1006/mcpr.2002.0410]

Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France

Botti S.;Bertaccini A.
2002

Abstract

Flavescence dorée is a devastating disease of grapevine widespread in several countries in EU such as France, Italy and Spain. Genetic variability among 17 Italian and 3 French FD strains was investigated by RFLP analyses based on a fragment of the ribosomal protein operon and on the non-ribosomal DNA fragment FD9. RFLP analysis of the PCR amplified ribosomal protein fragment, coding for the 3′ end of rp122 and the entire rps3 genes, differentiated 4 rp-subgroups among the FD strains and 4 subgroups among the reference strains belonging to elm yellows group (16SrV). Sequencing and phylogenetic analysis of the same ribosomal protein DNA fragment validated the delineation of 4 distinct FD strain types derived by RFLP analyses. The results supported the differentiation based on analysis of the non-ribosomal DNA fragment FD9. The phylogenetic analysis further revealed relationships and a probable evolutionary trend among the FD strains and the other representatives of elm yellows group. All the FD strains together with the reference strains ALY, RuS and JWB formed a cluster very well distinct from the EY/ULW cluster. Moreover, ALY was shown to be more closely related to three FD strain types: the Lombardia/Piemonte, the French FD70, and the French FD88/Italian FD-D strain clusters. © 2002 Elsevier Science Ltd. All rights reserved.
2002
Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France / Martini M.; Botti S.; Marcone C.; Marzachi C.; Casati P.; Bianco P.A.; Benedetti R.; Bertaccini A.. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - STAMPA. - 16:3(2002), pp. 197-208. [10.1006/mcpr.2002.0410]
Martini M.; Botti S.; Marcone C.; Marzachi C.; Casati P.; Bianco P.A.; Benedetti R.; Bertaccini A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/901842
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