This study evaluated the bioenergetic map of mitochondria metabolism in cryopreserved bovine sperm. The detected oligomycin-sensitive basal respiration supported ATP production; frozen-thawed spermatozoa were found to have a coupling efficiency higher than 0.80. Cell respiration, however, was not stimulated by the protonophoric action of FCCP, as its titration with 1, 2, 4 and 6 mu M did not stimulate the uncoupling activity on oxidative phosphorylation as highlighted by unresponsive oxygen consumption. The unusual effect on the stimulation of maximal respiration was not related to fibronectin- or PDL-coated plates used for cellular metabolism analysis. Conversely, irradiation of frozen-thawed bovine sperm with the red light improved mitochondrial parameters. In effect, the maximal respiration of red-light-stimulated sperm in PDL-coated plates was higher than the non-irradiated. In spite of this, red-light irradiation had no impact on membrane integrity and mitochondrial activity evaluated by epifluorescence microscopy.

Algieri, C., Blanco-Prieto, O., Llavanera, M., Yeste, M., Spinaci, M., Mari, G., et al. (2023). Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm. REPRODUCTION IN DOMESTIC ANIMALS, 58(1), 184-188 [10.1111/rda.14261].

Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm

Algieri, Cristina
Co-primo
;
Blanco-Prieto, Olga
Co-primo
;
Spinaci, Marcella;Mari, Gaetano;Bucci, Diego
;
Nesci, Salvatore
Ultimo
2023

Abstract

This study evaluated the bioenergetic map of mitochondria metabolism in cryopreserved bovine sperm. The detected oligomycin-sensitive basal respiration supported ATP production; frozen-thawed spermatozoa were found to have a coupling efficiency higher than 0.80. Cell respiration, however, was not stimulated by the protonophoric action of FCCP, as its titration with 1, 2, 4 and 6 mu M did not stimulate the uncoupling activity on oxidative phosphorylation as highlighted by unresponsive oxygen consumption. The unusual effect on the stimulation of maximal respiration was not related to fibronectin- or PDL-coated plates used for cellular metabolism analysis. Conversely, irradiation of frozen-thawed bovine sperm with the red light improved mitochondrial parameters. In effect, the maximal respiration of red-light-stimulated sperm in PDL-coated plates was higher than the non-irradiated. In spite of this, red-light irradiation had no impact on membrane integrity and mitochondrial activity evaluated by epifluorescence microscopy.
2023
Algieri, C., Blanco-Prieto, O., Llavanera, M., Yeste, M., Spinaci, M., Mari, G., et al. (2023). Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm. REPRODUCTION IN DOMESTIC ANIMALS, 58(1), 184-188 [10.1111/rda.14261].
Algieri, Cristina; Blanco-Prieto, Olga; Llavanera, Marc; Yeste, Marc; Spinaci, Marcella; Mari, Gaetano; Bucci, Diego; Nesci, Salvatore
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/901794
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