A recombinant clone was isolated at random from a skeletal muscle cDNA library. The sequence of the resulting 598 bp insert (EMBL accession number X94253) was compared in EMBL and GenBank databases using BLASTN2. The comparisons showed the highest matches with mouse and human PCBP2 cDNAs (mouse X75947, mouse X97982, human X78136 and mouse L19661; BLASTN probability were 1·9e-182, 1·1e-176, 1·6e-174 and 4·7 e-172, respectively). Excluding two gaps that could be due to alternative splicing, the deduced porcine protein sequence revealed the complete identity with corresponding human ( X78136) and mouse ( L19661) sequences. The mouse sequences X75947and X97982showed only one amino acid difference (Arg instead of Gly) with the deduced amino acid sequence of the porcine cDNA clone isolated. The cDNA was used as probe for in situ hybridization and for RFLP analysis in three generation reference populations. Both approaches assigned this gene to porcine chromosome 5.
Davoli R., Fontanesi L., Cepica S., Musilova P., Stratil A., Rubes J., et al. (1999). The porcine poly(rC)-binding protein 2 (PCBP2) gene maps to chromosome 5. ANIMAL GENETICS, 30(2), 165-166 [10.1046/j.1365-2052.1999.00382-7.x].
The porcine poly(rC)-binding protein 2 (PCBP2) gene maps to chromosome 5
Davoli R.;Fontanesi L.;Russo V.
1999
Abstract
A recombinant clone was isolated at random from a skeletal muscle cDNA library. The sequence of the resulting 598 bp insert (EMBL accession number X94253) was compared in EMBL and GenBank databases using BLASTN2. The comparisons showed the highest matches with mouse and human PCBP2 cDNAs (mouse X75947, mouse X97982, human X78136 and mouse L19661; BLASTN probability were 1·9e-182, 1·1e-176, 1·6e-174 and 4·7 e-172, respectively). Excluding two gaps that could be due to alternative splicing, the deduced porcine protein sequence revealed the complete identity with corresponding human ( X78136) and mouse ( L19661) sequences. The mouse sequences X75947and X97982showed only one amino acid difference (Arg instead of Gly) with the deduced amino acid sequence of the porcine cDNA clone isolated. The cDNA was used as probe for in situ hybridization and for RFLP analysis in three generation reference populations. Both approaches assigned this gene to porcine chromosome 5.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
