Linkage assignment of the fast skeletal alkali myosin light polypeptide 1 (MYL1) gene to porcine chromosome 15

Two classes of myosin light chains, the alkali light chains and the regulatory light chains, are associated with the head region of skeletal muscle myosin heavy chains, forming the functional myosin molecule. The fast skeletal alkali myosin light polypeptide 1 (MYL1) gene encodes two isoforms, 1F and 3F, by means of alternative splicing of primary transcripts originated from two different promoters. A porcine cDNA (EMBL accession number X94689), coding for the complete fast skeletal alkali myosin light chain 3F isoform and isolated from an adult porcine skeletal muscle cDNA library, was used as probe in RFLP analyses. A segregating polymorphism in three-generation porcine refernce families was used to linkage map this gene to porcine chromosome 15.