Covalent binding of 14C-1,1,1-trichloroethane to macromolecules from rat and mouse liver, kidney, lung and stomach was analyzed under the same experimental conditions previously utilized in studying 1,1-dichloroethane and 1,1,2-trichloroethane. Labeling of DNA, RNA and proteins was very low in in vivo interaction and in in vitro microsome-mediated binding. Interaction proceeded through the involvement of the P-450-dependent mixed function oxidase system from liver microsomes and, to a lesser extent, from lung microsomes. Covalent Binding Index, of 1,1,1-trichloroethane in liver DNA was typical of very weak initiators. However, overall evaluation of the short-term assays available for 1,1,1-trichloroethane leads to limited evidence of genotoxicity. On the other hand, the evidence of 1,1,1-trichloroethane carcinogenicity in animals is still inadequate.
Turina M.P., Colacci A., Grilli S., Mazzullo M., Prodi G., Lattanzi G. (1986). Short-term tests of genotoxicity for 1,1,1-trichloroethane. RESEARCH COMMUNICATIONS IN CHEMICAL PATHOLOGY AND PHARMACOLOGY, 52(3), 305-320.
Short-term tests of genotoxicity for 1,1,1-trichloroethane
Turina M. P.;Colacci A.;Grilli S.;Mazzullo M.;Prodi G.;
1986
Abstract
Covalent binding of 14C-1,1,1-trichloroethane to macromolecules from rat and mouse liver, kidney, lung and stomach was analyzed under the same experimental conditions previously utilized in studying 1,1-dichloroethane and 1,1,2-trichloroethane. Labeling of DNA, RNA and proteins was very low in in vivo interaction and in in vitro microsome-mediated binding. Interaction proceeded through the involvement of the P-450-dependent mixed function oxidase system from liver microsomes and, to a lesser extent, from lung microsomes. Covalent Binding Index, of 1,1,1-trichloroethane in liver DNA was typical of very weak initiators. However, overall evaluation of the short-term assays available for 1,1,1-trichloroethane leads to limited evidence of genotoxicity. On the other hand, the evidence of 1,1,1-trichloroethane carcinogenicity in animals is still inadequate.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.