A cDNA coding for alkali myosin light chain 3 (MLC3F) was isolated from a porcine skeletal muscle library. This clone has an insert of 859 bp encompassing the complete CDS (coding sequence) plus the 5′ and 3′ untranslated regions. Computer analysis showed that porcine MLC3F cDNA is highly homologous to the corresponding cDNAs of human, rabbit and rat. Moreover, Northern analysis showed the presence of two bands that represent the mature mRNAs of the MLC1F and MLC3F isoforms according to data observed in other species.
Isolation and sequencing of porcine fast skeletal muscle alkali myosin light chain 3 cDNA / Davoli R.; Fontanesi L.; Costosi E.; Zambonelli P.; Russo V.. - In: ANIMAL BIOTECHNOLOGY. - ISSN 1049-5398. - STAMPA. - 8:2(1997), pp. 179-185. [10.1080/10495399709525880]
Isolation and sequencing of porcine fast skeletal muscle alkali myosin light chain 3 cDNA
Davoli R.
Primo
;Fontanesi L.Secondo
;Zambonelli P.Penultimo
;Russo V.Ultimo
1997
Abstract
A cDNA coding for alkali myosin light chain 3 (MLC3F) was isolated from a porcine skeletal muscle library. This clone has an insert of 859 bp encompassing the complete CDS (coding sequence) plus the 5′ and 3′ untranslated regions. Computer analysis showed that porcine MLC3F cDNA is highly homologous to the corresponding cDNAs of human, rabbit and rat. Moreover, Northern analysis showed the presence of two bands that represent the mature mRNAs of the MLC1F and MLC3F isoforms according to data observed in other species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
