Topoisomerase 1–DNA cleavage complexes (Top1ccs) form at DNA sites of Top1 activity and are increased by a highly specific anticancer drug (camptothecin, CPT) in living cells. Various methods are available to detect Top1ccs in cultured cells, including protocols based on the use of specific antibodies. Here, we describe a protocol to isolate Top1ccs at high purity, which does not depend on antibodies.
Jessica Marinello, G.C. (2022). Highly Purified Top1-Bound DNA Fragments. New York : Humana Press Inc. [10.1007/978-1-0716-2477-7_13].
Highly Purified Top1-Bound DNA Fragments
Jessica Marinello;Giovanni Capranico
2022
Abstract
Topoisomerase 1–DNA cleavage complexes (Top1ccs) form at DNA sites of Top1 activity and are increased by a highly specific anticancer drug (camptothecin, CPT) in living cells. Various methods are available to detect Top1ccs in cultured cells, including protocols based on the use of specific antibodies. Here, we describe a protocol to isolate Top1ccs at high purity, which does not depend on antibodies.File in questo prodotto:
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