Supplementation of Boswellia serrata and Salix alba Extracts during the Early Laying Phase: Effects on Serum and Albumen Proteins, Trace Elements, and Yolk Cholesterol

Abstract: Extracts from Boswellia serrata (Bs) and Salix alba (Sa) are used as supplement in poultry feed. The aims of this research were to study possible effects of a dietary supplementation with Bs and Sa on serum and albumen proteins, zinc and iron, and yolk cholesterol content in Leg-horn hens during the critical phase of the onset of laying. A total of 120 pullets, 17 weeks of age, were assigned to 2 groups (Control (C) and Treated (T), n = 60 each). The T group received a diet supplemented with 0.3% of dry extracts of Bs (5%) and Sa (5%) for 12 weeks. The study lasted 19 weeks. Serum proteins were fractionated using agarose gel electrophoresis (AGE) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Trace elements were determined in serum using atomic absorption spectrometry and yolk cholesterol was determined using a colorimetric test. No significant differences were observed between control and supplemented hens for ana-lyzed biochemical indices. Moreover, the supplementation with phytoextracts did not negative-ly affect the physiological variations of serum proteins therefore it can be safely used as a treatment to prevent inflammatory states at onset and during the early laying phase.