An indirect competitive Chemiluminescence Enzyme-Linked Immunosorbent Assay (CLEIA) for determination of benzene, toluene, ethylbenzene, and ortho-, meta-, para-xylenes (BTEX) in soil and water was developed. The assay was optimized concerning coating conjugate concentration, anti-BTEX antiserum dilution, the incubation time effect on primary and secondary antibody incubation, and the temperature effect on competitive step and tolerance to different organic solvents. The IC50 and lower limit of detection (LDD90) values were 4.6 µg mL-1 and 0.5 µg mL -1, respectively. While water samples could be analyzed directly, soil has to be extracted and diluted prior to immunochemical measurement. BTEX could be recovered from spiked soil with a yield higher than 60% using 5-min ultrasonication with methanol. Finally, the assay was applied to soil and water samples collected at a contaminated site in Italy and was compared to GC-MS.

Chemiluminescent ELISA for BTEX determination in water and soil

MAIOLINI, ELISABETTA;BOLELLI, LUCA;FERRI, ELIDA NORA;GIROTTI, STEFANO
2010

Abstract

An indirect competitive Chemiluminescence Enzyme-Linked Immunosorbent Assay (CLEIA) for determination of benzene, toluene, ethylbenzene, and ortho-, meta-, para-xylenes (BTEX) in soil and water was developed. The assay was optimized concerning coating conjugate concentration, anti-BTEX antiserum dilution, the incubation time effect on primary and secondary antibody incubation, and the temperature effect on competitive step and tolerance to different organic solvents. The IC50 and lower limit of detection (LDD90) values were 4.6 µg mL-1 and 0.5 µg mL -1, respectively. While water samples could be analyzed directly, soil has to be extracted and diluted prior to immunochemical measurement. BTEX could be recovered from spiked soil with a yield higher than 60% using 5-min ultrasonication with methanol. Finally, the assay was applied to soil and water samples collected at a contaminated site in Italy and was compared to GC-MS.
E.Maiolini; D.Knopp; R.Niessner; S.Eremin; L.Bolelli; E.N.Ferri; S.Girotti
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/89225
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 7
social impact