The effects of Al3+ [supplied as Al2(SO4)3·18H2O] addition to culture media (pH 4.0) on growth, morphogenesis (in leaf explants), and oxidative stress reactions in in vitro cultures of ‘BA 29’ quince were investigated. Aluminium (Al 0.5mM) strongly inhibited shoot growth in the proliferation and rooting phases (Al 2.2 mM), reduced shoot proliferation (Al 1.1 mM), and induced tissue browning. Superoxide dismutase (SOD) activity was increased in shoot cultures supplemented with 2mMAl. Malondialdehyde (MDA) content of shoots was strongly increased by Al during proliferation (starting from Al 1.7mM) and rooting (already at Al 1.1 mM), thus serving as a good ‘marker’ for Al toxicity. Even a low concentration of Al (0.5mM) in the shoot induction medium was found to inhibit shoot regeneration. When standard (Al 0) shoot induction medium was used, leaf explant growth was only reduced by 2.2mM Al in the subsequent growth phases. Following a preliminary selection for their growth on Al-enriched media, 82 potentially Al-tolerant quince somaclones were selected for further trials.
M.W. Schuch, A. Cellini, A. Masia, G. Marino (2010). Aluminium-induced effects on growth, morphogenesis and oxidative stress reactions in in vitro cultures of quince. SCIENTIA HORTICULTURAE, 125, 151-158 [10.1016/j.scienta.2010.03.007].
Aluminium-induced effects on growth, morphogenesis and oxidative stress reactions in in vitro cultures of quince
CELLINI, ANTONIO;MASIA, ANDREA;MARINO, GRAZIA
2010
Abstract
The effects of Al3+ [supplied as Al2(SO4)3·18H2O] addition to culture media (pH 4.0) on growth, morphogenesis (in leaf explants), and oxidative stress reactions in in vitro cultures of ‘BA 29’ quince were investigated. Aluminium (Al 0.5mM) strongly inhibited shoot growth in the proliferation and rooting phases (Al 2.2 mM), reduced shoot proliferation (Al 1.1 mM), and induced tissue browning. Superoxide dismutase (SOD) activity was increased in shoot cultures supplemented with 2mMAl. Malondialdehyde (MDA) content of shoots was strongly increased by Al during proliferation (starting from Al 1.7mM) and rooting (already at Al 1.1 mM), thus serving as a good ‘marker’ for Al toxicity. Even a low concentration of Al (0.5mM) in the shoot induction medium was found to inhibit shoot regeneration. When standard (Al 0) shoot induction medium was used, leaf explant growth was only reduced by 2.2mM Al in the subsequent growth phases. Following a preliminary selection for their growth on Al-enriched media, 82 potentially Al-tolerant quince somaclones were selected for further trials.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
