The pretreatment of tobacco leaves with bacterial pr-LPS complexes induces after 48 h a tolerance state towards Pseudomonas syringae pv. aptata and prevents the hypersensitive confluent necrosis. TLC and HPLC were used to detect in the tobacco mesophyll induction of antimicrobial compounds (i.e. sesquiterpenoids or isoflavonoids) by pr-LPS treatment. One inhibition zone was detected by TLC bioassay with Cladosporium cucumerinum in the chloroform-soluble fraction of the tissue injected 48 h earlier with pr-LPS complexes. The nature and the role of this material has been discussed.
Detection of antimicrobial compounds in tobacco leaves protected by bacterial protein-lipopolysaccharides
paola minardi
1990
Abstract
The pretreatment of tobacco leaves with bacterial pr-LPS complexes induces after 48 h a tolerance state towards Pseudomonas syringae pv. aptata and prevents the hypersensitive confluent necrosis. TLC and HPLC were used to detect in the tobacco mesophyll induction of antimicrobial compounds (i.e. sesquiterpenoids or isoflavonoids) by pr-LPS treatment. One inhibition zone was detected by TLC bioassay with Cladosporium cucumerinum in the chloroform-soluble fraction of the tissue injected 48 h earlier with pr-LPS complexes. The nature and the role of this material has been discussed.File in questo prodotto:
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