A survey for the presence of aster yellows-related phytoplasmas in the different life stages of Scapholdeus titanus was carried out by means of PCR and nested-PCR assays. Using a phytoplasma 16Srl group-specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma-free Vicia faba seedlings. Two of these bread beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows-related phytoplasmas in S. titanus.
Alma A., Bosco D., Danielli A., Bertaccini A., Vibio M., Arzone A. (1997). Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants. INSECT MOLECULAR BIOLOGY, 6(2), 115-121 [10.1111/j.1365-2583.1997.tb00079.x].
Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants
Alma A.;Danielli A.;Bertaccini A.;Vibio M.;
1997
Abstract
A survey for the presence of aster yellows-related phytoplasmas in the different life stages of Scapholdeus titanus was carried out by means of PCR and nested-PCR assays. Using a phytoplasma 16Srl group-specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma-free Vicia faba seedlings. Two of these bread beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows-related phytoplasmas in S. titanus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
