Background and Objectives: The ligase chain reaction is an in vitro DNA amplification technique that exponentially amplifies selected DNA sequences. Goal: To evaluate a ligase chain reaction assay for the detection of Chlamydia trachomatis cryptic plasmid DNA (LCx Chlamydia) in patients routinely attending a sexually transmitted disease center in Italy. Study Design: Urethral or cervical swabs were obtained from 501 consecutive patients (334 men and 167 women). The samples were assayed in parallel with LCx Chlamydia and conventional tissue culture; discordant results were further assayed by direct immunofluorescence and a ligase chain reaction with alternate primers. Results: After resolution of discordant results, the LCx method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 99.3%, 96.7%, and 100% in men; 100%, 100%, 100%, and 100% in women; and 100%, 99.5%, 97.1%, and 100% overall, respectively. By comparison, the sensitivity of tissue culture was 81.4% in men, 50% in women, and 77.6% overall. Conclusions: The automated LCx method is sensitive, fast, and accurate and represents a useful diagnostic tool for C. trachomatis infection, even in low and medium prevalence populations.

Rumpianesi F., Donati M., Negosanti M., D'Antuono A., La Placa M., Cevenini R. (1996). Detection of Chlamydia trachomatis by a ligase chain reaction amplification method. SEXUALLY TRANSMITTED DISEASES, 23(3), 177-180 [10.1097/00007435-199605000-00003].

Detection of Chlamydia trachomatis by a ligase chain reaction amplification method

Negosanti M.;D'Antuono A.;La Placa M.;Cevenini R.
1996

Abstract

Background and Objectives: The ligase chain reaction is an in vitro DNA amplification technique that exponentially amplifies selected DNA sequences. Goal: To evaluate a ligase chain reaction assay for the detection of Chlamydia trachomatis cryptic plasmid DNA (LCx Chlamydia) in patients routinely attending a sexually transmitted disease center in Italy. Study Design: Urethral or cervical swabs were obtained from 501 consecutive patients (334 men and 167 women). The samples were assayed in parallel with LCx Chlamydia and conventional tissue culture; discordant results were further assayed by direct immunofluorescence and a ligase chain reaction with alternate primers. Results: After resolution of discordant results, the LCx method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 99.3%, 96.7%, and 100% in men; 100%, 100%, 100%, and 100% in women; and 100%, 99.5%, 97.1%, and 100% overall, respectively. By comparison, the sensitivity of tissue culture was 81.4% in men, 50% in women, and 77.6% overall. Conclusions: The automated LCx method is sensitive, fast, and accurate and represents a useful diagnostic tool for C. trachomatis infection, even in low and medium prevalence populations.
1996
Rumpianesi F., Donati M., Negosanti M., D'Antuono A., La Placa M., Cevenini R. (1996). Detection of Chlamydia trachomatis by a ligase chain reaction amplification method. SEXUALLY TRANSMITTED DISEASES, 23(3), 177-180 [10.1097/00007435-199605000-00003].
Rumpianesi F.; Donati M.; Negosanti M.; D'Antuono A.; La Placa M.; Cevenini R.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/885425
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