The bvg locus of Bordetella pertussis encodes an environmentally inducible operon essential for the expression of virulence genes. We show that in Escherichia coli, the P(TOX) promoter cloned in cis of the bvg locus is activated and environmentally regulated. Cotransformation of E. coli with the bvg locus cloned in a low-copy-number plasmid and with the P(TOX) promoter cloned in a high-copy-number plasmid can give rise to two different results. If the P(TOX) promoter is cloned in the pGem-3 vector, transcription is absent. If the P(TOX) promoter is cloned in the plasmid pKK232, containing the P(TOX) promoter between two ribosomal gene terminators of transcription, transcription occurs, although regulation of transcription is abolished. Under these conditions, the intracellular amount of RNA transcripts is increased by adding to the culture medium novobiocin, an inhibitor of bacterial gyrases. In vitro, the transcription of the P(TOX) promoter is activated on E. coli RNA polymerase supplemented with cell extracts from wild-type B. pertussis. Addition of DNA gyrase to the mixture dramatically reduces the amount of RNA synthesized. Our data show that the products of the bvg locus, BvgA and BvgS, are directly involved in the regulation of the P(TOX) promoter in E. coli and that DNA topology may play a role in the induction of transcription.

Scarlato V., Arico B., Rappuoli R. (1993). DNA topology affects transcriptional regulation of the pertussis toxin gene of Bordetella pertussis in Escherichia coli and in vitro. JOURNAL OF BACTERIOLOGY, 175(15), 4764-4771 [10.1128/jb.175.15.4764-4771.1993].

DNA topology affects transcriptional regulation of the pertussis toxin gene of Bordetella pertussis in Escherichia coli and in vitro

Scarlato V.;
1993

Abstract

The bvg locus of Bordetella pertussis encodes an environmentally inducible operon essential for the expression of virulence genes. We show that in Escherichia coli, the P(TOX) promoter cloned in cis of the bvg locus is activated and environmentally regulated. Cotransformation of E. coli with the bvg locus cloned in a low-copy-number plasmid and with the P(TOX) promoter cloned in a high-copy-number plasmid can give rise to two different results. If the P(TOX) promoter is cloned in the pGem-3 vector, transcription is absent. If the P(TOX) promoter is cloned in the plasmid pKK232, containing the P(TOX) promoter between two ribosomal gene terminators of transcription, transcription occurs, although regulation of transcription is abolished. Under these conditions, the intracellular amount of RNA transcripts is increased by adding to the culture medium novobiocin, an inhibitor of bacterial gyrases. In vitro, the transcription of the P(TOX) promoter is activated on E. coli RNA polymerase supplemented with cell extracts from wild-type B. pertussis. Addition of DNA gyrase to the mixture dramatically reduces the amount of RNA synthesized. Our data show that the products of the bvg locus, BvgA and BvgS, are directly involved in the regulation of the P(TOX) promoter in E. coli and that DNA topology may play a role in the induction of transcription.
1993
Scarlato V., Arico B., Rappuoli R. (1993). DNA topology affects transcriptional regulation of the pertussis toxin gene of Bordetella pertussis in Escherichia coli and in vitro. JOURNAL OF BACTERIOLOGY, 175(15), 4764-4771 [10.1128/jb.175.15.4764-4771.1993].
Scarlato V.; Arico B.; Rappuoli R.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/882531
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 17
social impact