We report a self-splicing intron in bacteriophage SPO1, whose host is the gram-positive Bacillus subtilis. The intron contains all the conserved features of primary sequence and secondary structure previously described for the group IA introns of eukaryotic organelles and the gram-negative bacteriophage T4. The SPO1 intron contains an open reading frame of 522 nucleotides. As in the T4 introns, this open reading frame begins in a region that is looped out of the secondary structure, but ends in a highly conserved region of the intron core. The exons encode SPO1 DNA polymerase, which is highly similar to E. coli DNA polymerase I. The demonstration of self-splicing introns in viruses of both gram-positive and gramnegative eubacteria lends further evidence for their early origin in evolution. © 1990.
Goodrich-Blair H., Scarlato V., Gott J.M., Xu M.-Q., Shub D.A. (1990). A self-splicing group I intron in the DNA polymerase gene of bacillus subtilis bacteriophage SPO1. CELL, 63(2), 417-424 [10.1016/0092-8674(90)90174-D].
A self-splicing group I intron in the DNA polymerase gene of bacillus subtilis bacteriophage SPO1
Scarlato V.;
1990
Abstract
We report a self-splicing intron in bacteriophage SPO1, whose host is the gram-positive Bacillus subtilis. The intron contains all the conserved features of primary sequence and secondary structure previously described for the group IA introns of eukaryotic organelles and the gram-negative bacteriophage T4. The SPO1 intron contains an open reading frame of 522 nucleotides. As in the T4 introns, this open reading frame begins in a region that is looped out of the secondary structure, but ends in a highly conserved region of the intron core. The exons encode SPO1 DNA polymerase, which is highly similar to E. coli DNA polymerase I. The demonstration of self-splicing introns in viruses of both gram-positive and gramnegative eubacteria lends further evidence for their early origin in evolution. © 1990.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
