We report a self-splicing intron in bacteriophage SPO1, whose host is the gram-positive Bacillus subtilis. The intron contains all the conserved features of primary sequence and secondary structure previously described for the group IA introns of eukaryotic organelles and the gram-negative bacteriophage T4. The SPO1 intron contains an open reading frame of 522 nucleotides. As in the T4 introns, this open reading frame begins in a region that is looped out of the secondary structure, but ends in a highly conserved region of the intron core. The exons encode SPO1 DNA polymerase, which is highly similar to E. coli DNA polymerase I. The demonstration of self-splicing introns in viruses of both gram-positive and gramnegative eubacteria lends further evidence for their early origin in evolution. © 1990.

A self-splicing group I intron in the DNA polymerase gene of bacillus subtilis bacteriophage SPO1

Scarlato V.;
1990

Abstract

We report a self-splicing intron in bacteriophage SPO1, whose host is the gram-positive Bacillus subtilis. The intron contains all the conserved features of primary sequence and secondary structure previously described for the group IA introns of eukaryotic organelles and the gram-negative bacteriophage T4. The SPO1 intron contains an open reading frame of 522 nucleotides. As in the T4 introns, this open reading frame begins in a region that is looped out of the secondary structure, but ends in a highly conserved region of the intron core. The exons encode SPO1 DNA polymerase, which is highly similar to E. coli DNA polymerase I. The demonstration of self-splicing introns in viruses of both gram-positive and gramnegative eubacteria lends further evidence for their early origin in evolution. © 1990.
Goodrich-Blair H.; Scarlato V.; Gott J.M.; Xu M.-Q.; Shub D.A.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/882515
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