The bacteriophage SP01 gene 30, whose function is essential for DNA synthesis, has been analyzed for its primary structural features. Conditionally lethal mutations in the gene 30 locus have been mapped and sequenced, and the wild-type amino acid (aa) sequence has been deduced along with that of a co-transcribed and possibly co-translated upstream unidentified reading frame (URF). The aa sequence deduced for gene 30 shares partial similarity with protein P of bacteriophage λ, which participated in λ DNA replication, and also with the exonuclease, gp46, of bacteriophage T4. A lysine-rich region of the hypothetical product of the URF shares similarity with both the T4 DNA topoisomerase and the θ29 gene 3-encoded protein; the latter codes for a terminal protein which participates in the priming of DNA elongation. © 1992.

Scarlato V., Sayre M.H. (1992). Sequence of the bacteriophage SP01 gene 30. GENE, 114(1), 115-119 [10.1016/0378-1119(92)90716-3].

Sequence of the bacteriophage SP01 gene 30

Scarlato V.;
1992

Abstract

The bacteriophage SP01 gene 30, whose function is essential for DNA synthesis, has been analyzed for its primary structural features. Conditionally lethal mutations in the gene 30 locus have been mapped and sequenced, and the wild-type amino acid (aa) sequence has been deduced along with that of a co-transcribed and possibly co-translated upstream unidentified reading frame (URF). The aa sequence deduced for gene 30 shares partial similarity with protein P of bacteriophage λ, which participated in λ DNA replication, and also with the exonuclease, gp46, of bacteriophage T4. A lysine-rich region of the hypothetical product of the URF shares similarity with both the T4 DNA topoisomerase and the θ29 gene 3-encoded protein; the latter codes for a terminal protein which participates in the priming of DNA elongation. © 1992.
1992
Scarlato V., Sayre M.H. (1992). Sequence of the bacteriophage SP01 gene 30. GENE, 114(1), 115-119 [10.1016/0378-1119(92)90716-3].
Scarlato V.; Sayre M.H.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/882489
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