Bacterial strains belonging to the genus Rhodococcus are capable to degrade various toxic organic compounds and tolerate high concentrations of metal(loid)s. We have previously shown that R. aetherivorans BCP1 is resistant to various levels of the two arsenic inorganic species, arsenite [As(III)] and arsenate [As(V)]. However, while arsenite showed toxic effects as early as 5 mM, arsenate at 30 mM even boosted the growth rate of BCP1 cells and was only toxic at concentrations > 100 mM. Since such behavior could be linked to peculiar aspects of its metabolism, the transcriptomic analysis of BCP1 cells exposed to 5 mM As(III) and 30 mM As(V), was performed in this work. The aim was to clarify the mechanisms underlying the arsenic stress response of the two growth phenotypes in the presence of the two different oxyanions. The results revealed that As(III) induced higher activity of ROS-scavenging enzymes as compared to As(V) in relation with the expression of enzymes involved in cellular damage recovery and redox buffers/cofactors (ergothioneine, mycofactocin and mycothiol). Further, As(III) downregulated pathways related to cell division while both oxyanions downregulated genes involved in glycolysis. Notably, As(V) induced the expression of enzymes participating in the synthesis of metallophores and rearranged the central and energetic metabolism, also inducing alternative pathways for ATP synthesis and glucose consumption. This study, in providing the transcriptomic data on R. aetherivorans exposed to arsenic oxyanions, sheds some light on the plasticity of rhodococcal response to arsenic stress, which may be important for the improvement of biotechnological applications.

Transcriptomic analysis of the dual response of Rhodococcus aetherivorans BCP1 to inorganic arsenic oxyanions / Andrea Firrincieli, Davide Zannoni, Eva Donini, Hana Dostálová, Robert Rädisch, Luisa Iommarini, Raymond Turner, Tobias Busche, Miroslav Pátek, Martina Cappelletti. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 1098-5336. - ELETTRONICO. - 88:7(2022), pp. 1-11. [10.1128/aem.02209-21]

Transcriptomic analysis of the dual response of Rhodococcus aetherivorans BCP1 to inorganic arsenic oxyanions

Andrea Firrincieli;Davide Zannoni;Eva Donini;Luisa Iommarini;Martina Cappelletti
2022

Abstract

Bacterial strains belonging to the genus Rhodococcus are capable to degrade various toxic organic compounds and tolerate high concentrations of metal(loid)s. We have previously shown that R. aetherivorans BCP1 is resistant to various levels of the two arsenic inorganic species, arsenite [As(III)] and arsenate [As(V)]. However, while arsenite showed toxic effects as early as 5 mM, arsenate at 30 mM even boosted the growth rate of BCP1 cells and was only toxic at concentrations > 100 mM. Since such behavior could be linked to peculiar aspects of its metabolism, the transcriptomic analysis of BCP1 cells exposed to 5 mM As(III) and 30 mM As(V), was performed in this work. The aim was to clarify the mechanisms underlying the arsenic stress response of the two growth phenotypes in the presence of the two different oxyanions. The results revealed that As(III) induced higher activity of ROS-scavenging enzymes as compared to As(V) in relation with the expression of enzymes involved in cellular damage recovery and redox buffers/cofactors (ergothioneine, mycofactocin and mycothiol). Further, As(III) downregulated pathways related to cell division while both oxyanions downregulated genes involved in glycolysis. Notably, As(V) induced the expression of enzymes participating in the synthesis of metallophores and rearranged the central and energetic metabolism, also inducing alternative pathways for ATP synthesis and glucose consumption. This study, in providing the transcriptomic data on R. aetherivorans exposed to arsenic oxyanions, sheds some light on the plasticity of rhodococcal response to arsenic stress, which may be important for the improvement of biotechnological applications.
2022
Transcriptomic analysis of the dual response of Rhodococcus aetherivorans BCP1 to inorganic arsenic oxyanions / Andrea Firrincieli, Davide Zannoni, Eva Donini, Hana Dostálová, Robert Rädisch, Luisa Iommarini, Raymond Turner, Tobias Busche, Miroslav Pátek, Martina Cappelletti. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 1098-5336. - ELETTRONICO. - 88:7(2022), pp. 1-11. [10.1128/aem.02209-21]
Andrea Firrincieli, Davide Zannoni, Eva Donini, Hana Dostálová, Robert Rädisch, Luisa Iommarini, Raymond Turner, Tobias Busche, Miroslav Pátek, Martina Cappelletti
File in questo prodotto:
File Dimensione Formato  
aem.02209-21.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 2.88 MB
Formato Adobe PDF
2.88 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/879504
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 3
social impact