Immunotoxins have been prepared with 3 ribosome-inactivating proteins (RIPs), namely, momordin, pokeweed anti-viral protein from seeds (PAP-S) and saporin, linked to the Ber-H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPs or the immunotoxins induced apoptosis in the CD30+ L540 cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidium-iodide staining and fluoresceine-isothiocyanate conjugate dUTP incorporation). The AC50 (concentration causing apoptosis in 50% of the cells) is in the range 10-8 to 10-7 M in the case of pips, and 10-11 to 10-10 M in the case of the immunotoxins.
Bolognesi A., Tazzari P.L., Olivieri F., Polito L., Falini B., Stirpe F. (1996). Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins. INTERNATIONAL JOURNAL OF CANCER, 68(3), 349-355 [10.1002/(SICI)1097-0215(19961104)68:3<349::AID-IJC13>3.0.CO;2-3].
Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins
Bolognesi A.Primo
;Polito L.;Stirpe F.Ultimo
1996
Abstract
Immunotoxins have been prepared with 3 ribosome-inactivating proteins (RIPs), namely, momordin, pokeweed anti-viral protein from seeds (PAP-S) and saporin, linked to the Ber-H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPs or the immunotoxins induced apoptosis in the CD30+ L540 cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidium-iodide staining and fluoresceine-isothiocyanate conjugate dUTP incorporation). The AC50 (concentration causing apoptosis in 50% of the cells) is in the range 10-8 to 10-7 M in the case of pips, and 10-11 to 10-10 M in the case of the immunotoxins.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
