Immunotoxins have been prepared with 3 ribosome-inactivating proteins (RIPs), namely, momordin, pokeweed anti-viral protein from seeds (PAP-S) and saporin, linked to the Ber-H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPs or the immunotoxins induced apoptosis in the CD30+ L540 cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidium-iodide staining and fluoresceine-isothiocyanate conjugate dUTP incorporation). The AC50 (concentration causing apoptosis in 50% of the cells) is in the range 10-8 to 10-7 M in the case of pips, and 10-11 to 10-10 M in the case of the immunotoxins.

Bolognesi A., Tazzari P.L., Olivieri F., Polito L., Falini B., Stirpe F. (1996). Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins. INTERNATIONAL JOURNAL OF CANCER, 68(3), 349-355 [10.1002/(SICI)1097-0215(19961104)68:3<349::AID-IJC13>3.0.CO;2-3].

Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins

Bolognesi A.
Primo
;
Polito L.;Stirpe F.
Ultimo
1996

Abstract

Immunotoxins have been prepared with 3 ribosome-inactivating proteins (RIPs), namely, momordin, pokeweed anti-viral protein from seeds (PAP-S) and saporin, linked to the Ber-H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPs or the immunotoxins induced apoptosis in the CD30+ L540 cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidium-iodide staining and fluoresceine-isothiocyanate conjugate dUTP incorporation). The AC50 (concentration causing apoptosis in 50% of the cells) is in the range 10-8 to 10-7 M in the case of pips, and 10-11 to 10-10 M in the case of the immunotoxins.
1996
Bolognesi A., Tazzari P.L., Olivieri F., Polito L., Falini B., Stirpe F. (1996). Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins. INTERNATIONAL JOURNAL OF CANCER, 68(3), 349-355 [10.1002/(SICI)1097-0215(19961104)68:3<349::AID-IJC13>3.0.CO;2-3].
Bolognesi A.; Tazzari P.L.; Olivieri F.; Polito L.; Falini B.; Stirpe F.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/877150
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