Bothrops jararaca Snake venom extract has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in order to obtain a rapid and accurate characterization. By the SDS-PAGE and MALDI-MS techniques about fourteen components are evidenced; with the latter technique, faster analysis and a more accurate molecular weight determination have been achieved. By ESI-MS the data obtained strongly depend on the operating conditions employed.

A comparison of the analytical performance of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrospray and matrix-assisted laser desorption/ionization mass spectrometry in the study of the protein extract from bothrops jararaca snake venom / Roda A.; Gioacchini A.M.; Seraglia R.; Montagnani M.; Baraldini M.; Pedrazzini S.; Puricelli M.; Traldi P.. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - STAMPA. - 11:12(1997), pp. 1297-1302. [10.1002/(SICI)1097-0231(199708)11:12<1297::AID-RCM963>3.0.CO;2-A]

A comparison of the analytical performance of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrospray and matrix-assisted laser desorption/ionization mass spectrometry in the study of the protein extract from bothrops jararaca snake venom

Roda A.;Montagnani M.;Baraldini M.;Puricelli M.;
1997

Abstract

Bothrops jararaca Snake venom extract has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in order to obtain a rapid and accurate characterization. By the SDS-PAGE and MALDI-MS techniques about fourteen components are evidenced; with the latter technique, faster analysis and a more accurate molecular weight determination have been achieved. By ESI-MS the data obtained strongly depend on the operating conditions employed.
1997
A comparison of the analytical performance of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrospray and matrix-assisted laser desorption/ionization mass spectrometry in the study of the protein extract from bothrops jararaca snake venom / Roda A.; Gioacchini A.M.; Seraglia R.; Montagnani M.; Baraldini M.; Pedrazzini S.; Puricelli M.; Traldi P.. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - STAMPA. - 11:12(1997), pp. 1297-1302. [10.1002/(SICI)1097-0231(199708)11:12<1297::AID-RCM963>3.0.CO;2-A]
Roda A.; Gioacchini A.M.; Seraglia R.; Montagnani M.; Baraldini M.; Pedrazzini S.; Puricelli M.; Traldi P.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/876634
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