A comparison of the analytical performance of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrospray and matrix-assisted laser desorption/ionization mass spectrometry in the study of the protein extract from bothrops jararaca snake venom

Roda, A.; Gioacchini, A. M.; Seraglia, R.; Montagnani, M.; Baraldini, M.; Pedrazzini, S.; Puricelli, M.; Traldi, P.

doi:10.1002/(SICI)1097-0231(199708)11:12<1297::AID-RCM963>3.0.CO;2-A

Bothrops jararaca Snake venom extract has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in order to obtain a rapid and accurate characterization. By the SDS-PAGE and MALDI-MS techniques about fourteen components are evidenced; with the latter technique, faster analysis and a more accurate molecular weight determination have been achieved. By ESI-MS the data obtained strongly depend on the operating conditions employed.

Titolo:	A comparison of the analytical performance of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, electrospray and matrix-assisted laser desorption/ionization mass spectrometry in the study of the protein extract from bothrops jararaca snake venom
Autore/i:	Roda A.; Gioacchini A. M.; Seraglia R.; Montagnani M.; Baraldini M.; Pedrazzini S.; Puricelli M.; Traldi P.
Autore/i Unibo:	RODA, ALDO Gioacchini A. M. Seraglia R. MONTAGNANI, MARCO BARALDINI, MARIO Pedrazzini S. PURICELLI, MARINA CARMELA L. Traldi P. mostra contributor esterni nascondi contributor esterni
Anno:	1997
Rivista:	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Digital Object Identifier (DOI):	http://dx.doi.org/10.1002/(SICI)1097-0231(199708)11:12<1297::AID-RCM963>3.0.CO;2-A
Abstract:	Bothrops jararaca Snake venom extract has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) electrospray mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in order to obtain a rapid and accurate characterization. By the SDS-PAGE and MALDI-MS techniques about fourteen components are evidenced; with the latter technique, faster analysis and a more accurate molecular weight determination have been achieved. By ESI-MS the data obtained strongly depend on the operating conditions employed.
Data stato definitivo:	2022-03-01T20:07:04Z
Appare nelle tipologie:	1.01 Articolo in rivista

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