A TILLING (Targeting Induced Local Lesion IN Genomes) resource in barley (cv. Morex) consisting of 4,906 families was produced by sodium azide (NaN_3 ) seed treatment. This resource has been named TILLMore. TILLMore was screened for mutants at several genes based on the analysis of 8- to 12-fold DNA pools produced from M2 or M3 DNA samples. An average of ca. six alleles per gene was identified, which corresponds to a rate of one mutation every 428 kb. Almost all the mutations detected were CG-TA transitions and several (ca. 58%) implied a change in amino acid sequence, hence possible effects on phenotype. The barley-mutagenized population, although developed for reverse-genetics purposes, is also a valuable resource for forward-genetics studies. A high frequency of M3 families (ca. 33%) showed morphological alterations, which have been scored regularly during the growing seasons in reference to wild-type Morex plants (see www.distagenomics.unibo.it/TILLMore/). In particular, a preliminary screening for phenotypes at the root level showed altered root morphology for ca. 7% of the families. Our results indicate the feasibility of using this collection of materials to investigate gene function in barley and closely related crops.
TALAME' V., BOVINA R., SALVI S., SANGUINETI M.C., PIFFANELLI P., LUNDQUIST U., et al. (2009). TILLING with TILLMore. ROMA : FAO.
TILLING with TILLMore
TALAME', VALENTINA;BOVINA, RICCARDO;SALVI, SILVIO;SANGUINETI, MARIA CORINNA;TUBEROSA, ROBERTO
2009
Abstract
A TILLING (Targeting Induced Local Lesion IN Genomes) resource in barley (cv. Morex) consisting of 4,906 families was produced by sodium azide (NaN_3 ) seed treatment. This resource has been named TILLMore. TILLMore was screened for mutants at several genes based on the analysis of 8- to 12-fold DNA pools produced from M2 or M3 DNA samples. An average of ca. six alleles per gene was identified, which corresponds to a rate of one mutation every 428 kb. Almost all the mutations detected were CG-TA transitions and several (ca. 58%) implied a change in amino acid sequence, hence possible effects on phenotype. The barley-mutagenized population, although developed for reverse-genetics purposes, is also a valuable resource for forward-genetics studies. A high frequency of M3 families (ca. 33%) showed morphological alterations, which have been scored regularly during the growing seasons in reference to wild-type Morex plants (see www.distagenomics.unibo.it/TILLMore/). In particular, a preliminary screening for phenotypes at the root level showed altered root morphology for ca. 7% of the families. Our results indicate the feasibility of using this collection of materials to investigate gene function in barley and closely related crops.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
