In the presence of Ca2+, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.
Purification of functional F-ATP synthase from blue native PAGE / Galber C.; Valente G.; von Stockum S.; Giorgio V.. - ELETTRONICO. - 1925:(2019), pp. 233-243. [10.1007/978-1-4939-9018-4_20]
Purification of functional F-ATP synthase from blue native PAGE
Giorgio V.
Ultimo
Funding Acquisition
2019
Abstract
In the presence of Ca2+, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.File in questo prodotto:
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