In the presence of Ca2+, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.
Galber C., Valente G., von Stockum S., Giorgio V. (2019). Purification of functional F-ATP synthase from blue native PAGE, 1925, 233-243 [10.1007/978-1-4939-9018-4_20].
Purification of functional F-ATP synthase from blue native PAGE
Giorgio V.
Ultimo
Funding Acquisition
2019
Abstract
In the presence of Ca2+, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.File in questo prodotto:
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