The involvement of the extracellular matrix (ECM) in lesion evolution and functional outcome is well recognized in spinal cord injury. Most attention has been dedicated to the “core” area of the lesion and scar formation, while only scattered reports consider ECM modification based on the temporal evolution and the segments adjacent to the lesion. In this study, we investigated the expression profile of 100 genes encoding for ECM proteins at 1, 8 and 45 days post-injury, in the spinal cord segments rostral and caudal to the lesion and in the scar segment, in a rat model. During both the active lesion phases and the lesion stabilization, we observed an asymmetric gene expression induced by the injury, with a higher regulation in the rostral segment of genes involved in ECM remodeling, adhesion and cell migration. Using bioinformatic approaches, the metalloproteases in-hibitor Timp1 and the hyaluronan receptor Cd44 emerged as the hub genes at all post-lesion times. Results from the bioinformatic gene expression analysis were then confirmed at protein level by tissue analysis and by cell culture using primary astrocytes. These results indicated that ECM regulation also takes place outside of the lesion area in spinal cord injury.

Bighinati A., Khalajzeyqami Z., Baldassarro V.A., Lorenzini L., Cescatti M., Moretti M., et al. (2021). Time-course changes of extracellular matrix encoding genes expression level in the spinal cord following contusion injury—a data-driven approach. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4), 1-20 [10.3390/ijms22041744].

Time-course changes of extracellular matrix encoding genes expression level in the spinal cord following contusion injury—a data-driven approach

Bighinati A.;Khalajzeyqami Z.;Baldassarro V. A.;Lorenzini L.;Cescatti M.;Moretti M.;Giardino L.;Calza Laura
Ultimo
2021

Abstract

The involvement of the extracellular matrix (ECM) in lesion evolution and functional outcome is well recognized in spinal cord injury. Most attention has been dedicated to the “core” area of the lesion and scar formation, while only scattered reports consider ECM modification based on the temporal evolution and the segments adjacent to the lesion. In this study, we investigated the expression profile of 100 genes encoding for ECM proteins at 1, 8 and 45 days post-injury, in the spinal cord segments rostral and caudal to the lesion and in the scar segment, in a rat model. During both the active lesion phases and the lesion stabilization, we observed an asymmetric gene expression induced by the injury, with a higher regulation in the rostral segment of genes involved in ECM remodeling, adhesion and cell migration. Using bioinformatic approaches, the metalloproteases in-hibitor Timp1 and the hyaluronan receptor Cd44 emerged as the hub genes at all post-lesion times. Results from the bioinformatic gene expression analysis were then confirmed at protein level by tissue analysis and by cell culture using primary astrocytes. These results indicated that ECM regulation also takes place outside of the lesion area in spinal cord injury.
2021
Bighinati A., Khalajzeyqami Z., Baldassarro V.A., Lorenzini L., Cescatti M., Moretti M., et al. (2021). Time-course changes of extracellular matrix encoding genes expression level in the spinal cord following contusion injury—a data-driven approach. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4), 1-20 [10.3390/ijms22041744].
Bighinati A.; Khalajzeyqami Z.; Baldassarro V.A.; Lorenzini L.; Cescatti M.; Moretti M.; Giardino L.; Calza Laura
File in questo prodotto:
File Dimensione Formato  
2021 - Bighinati - Int J Mol Sci.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 3.1 MB
Formato Adobe PDF
3.1 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/863585
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact