Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis.

Zangheri, M., Di Nardo, F., Calabria, D., Marchegiani, E., Anfossi, L., Guardigli, M., et al. (2021). Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee. ANALYTICA CHIMICA ACTA, 1163, 1-10 [10.1016/j.aca.2021.338515].

Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee

Zangheri, Martina
Primo
;
Calabria, Donato;Marchegiani, Elisa;Guardigli, Massimo;Mirasoli, Mara;Roda, Aldo
Ultimo
2021

Abstract

Exposure to mycotoxins, which may contaminate food and feed commodities, represents a serious health risk for consumers. Ochratoxin A (OTA) is one of the most abundant and toxic mycotoxins, thus specific regulations for fixing its maximum admissible levels in foodstuff have been established. Lateral Flow ImmunoAssay (LFIA)-based devices have been proposed as screening tools to avoid OTA contamination along the whole food chain. We report a portable, user-friendly smartphone-based biosensor for the detection and quantification of OTA in wine and instant coffee, which combines the LFIA approach with chemiluminescence (CL) detection. The device employs the smartphone camera as a light detector and uses low-cost, disposable analytical cartridges containing the LFIA strip and all the necessary reagents. The analysis can be carried out at the point of need by non-specialized operators through simple manual operations. The biosensor allows OTA quantitative detection in wine and coffee samples up to 25 μg L−1 and with limits of detection of 0.3 and 0.1 μg L−1, respectively, which are below the European law-fixed limits. These results demonstrate that the developed device can be used for routine monitoring of OTA contamination, enabling rapid and reliable identification of positive samples requiring confirmatory analysis.
2021
Zangheri, M., Di Nardo, F., Calabria, D., Marchegiani, E., Anfossi, L., Guardigli, M., et al. (2021). Smartphone biosensor for point-of-need chemiluminescence detection of ochratoxin A in wine and coffee. ANALYTICA CHIMICA ACTA, 1163, 1-10 [10.1016/j.aca.2021.338515].
Zangheri, Martina; Di Nardo, Fabio; Calabria, Donato; Marchegiani, Elisa; Anfossi, Laura; Guardigli, Massimo; Mirasoli, Mara; Baggiani, Claudio; Roda,...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/863418
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